ArticleMolecular and cellular biochemistry1997
Ribonuclease activity dependent cytotoxicity of Asp fl, a major allergen of A. fumigatus.
Article in Molecular and cellular biochemistry, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Modulation of Virulence-Associated Traits inMicroorganisms · 2022Article
- Aflatoxins: Implications on Health.Indian journal of clinical biochemistry : IJCB · 2017Review
- Microbial ribonucleases (RNases): production and application potential.World journal of microbiology & biotechnology · 2015Review
- Investigation of Aspergillus fumigatus biofilm formation by various "omics" approaches.Frontiers in microbiology · 2013Article
- Allergens/Antigens, toxins and polyketides of important Aspergillus species.Indian journal of clinical biochemistry : IJCB · 2011Article
- Prophylactic and Therapeutic Potential of Asp f1 Epitopes in Naïve and Sensitized BALB/c Mice.Immune network · 2009Article
- Tryptophan residue is essential for immunoreactivity of a diagnostically relevant peptide epitope of A. fumigatus.Molecular and cellular biochemistry · 2005Article
- Use of a synthetic peptide epitope of Asp f 1, a major allergen or antigen of Aspergillus fumigatus, for improved immunodiagnosis of allergic bronchopulmonary aspergillosis.Clinical and diagnostic laboratory immunology · 2004Article
- Expression and characterization of Asp fI, an immunodominant allergen/antigen of A. fumigatus in insect cell.Molecular and cellular biochemistry · 2003Article
- Host pathogen interactions in allergic and invasive fungal diseases: Diagnostic and therapeutic implications.Indian journal of clinical biochemistry : IJCB · 2001Article
- Challenges in prevention, diagnosis and therapy of emerging fungal diseases. Aspergillosis: A case study.Indian journal of clinical biochemistry : IJCB · 2000Article
- Biochemical characterization of an immunodominant allergen/antigen ofAspergillus fumigatus.Indian journal of clinical biochemistry : IJCB · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major allergen/antigen, Asp fl, secreted by Aspergillus fumigatus exhibits cytotoxicity towards eukaryotic cell lines. Asp fl inhibited protein synthesis in RAW cells with an IC50 of 4.5 nM and also degraded ribosomal RNA of RAW cells at a similar concentration. Ribosomal inactivation by Asp fl may be the probable mechanism for protein synthesis inhibition. Specific ribonuclease activity of Asp fl was observed to be 100,000 U/mg. Presence of strong RNase activity in Asp fl was further confirmed by agar gels containing yeast RNA. Electrophoretic run on agarose gels showed that Asp fl degrades all species of naked RNA. Modification of histidine residues of Asp fl with diethyl pyrocarbonate and alkylation of cysteines with iodoacetamide resulted in loss of ribonuclease activity and cytotoxicity of Asp fl. The current study establishes the ribonuclease activity of a purified major allergen of A. fumigatus that inhibits protein synthesis and kills the eukaryotic cells.
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