ReviewEnvironmental health perspectives1997
Artifacts associated with the measurement of oxidized DNA bases.
Review in Environmental health perspectives, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Why the ROS matters: One-electron oxidants focus DNA damage and repair on G-quadruplexes for gene regulation.DNA repair · 2025Review
- Pioneering contribution of Professor Bruce Ames to early development in biochemical aspects of oxidatively generated damage to DNA.Frontiers in molecular biosciences · 2025Review
- Detection of Oxidatively Modified Base Lesion(s) in Defined DNA Sequences by FLARE Quantitative PCR.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Innovative approaches for cancer treatment: current perspectives and new challenges.Ecancermedicalscience · 2019Article
- Solid-State Nanopore Analysis of Diverse DNA Base Modifications Using a Modular Enzymatic Labeling Process.Nano letters · 2017Article
- Formation and processing of DNA damage substrates for the hNEIL enzymes.Free radical biology & medicine · 2017Review
- Formation and repair of oxidatively generated damage in cellular DNA.Free radical biology & medicine · 2017Review
- Advanced Collaborative Emissions Study Auxiliary Findings on 2007-Compliant Diesel Engines: A Comparison With Diesel Exhaust Genotoxicity Effects Prior to 2007.Environmental health insights · 2017Review
- Stress-induced DNA damage biomarkers: applications and limitations.Frontiers in chemistry · 2015Review
- Radiation-mediated formation of complex damage to DNA: a chemical aspect overview.The British journal of radiology · 2014Review
- Detection and quantification of 8-hydroxy-2'-deoxyguanosine in Alzheimer's transgenic mouse urine using capillary electrophoresis.Electrophoresis · 2013Article
- Novel redox-sensing modules: accessory protein- and nucleic acid-mediated signaling.Antioxidants & redox signaling · 2012Review
- Site-specific radical formation in DNA induced by Cu(II)-H₂O₂ oxidizing system, using ESR, immuno-spin trapping, LC-MS, and MS/MS.Free radical biology & medicine · 2011Article
- Comparison of three oxidative stress biomarkers in a sample of healthy adults.Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals · 2009Article
- The PPARgamma agonist pioglitazone ameliorates aging-related progressive renal injury.Journal of the American Society of Nephrology : JASN · 2009Article
- Immunological detection of N-formylkynurenine in oxidized proteins.Free radical biology & medicine · 2009Article
- Oxidative-dependent integration of signal transduction with intercellular gap junctional communication in the control of gene expression.Antioxidants & redox signaling · 2009Review
- Pyrimido[1,2-a]-purin-10(3H)-one, M1G, is less prone to artifact than base oxidation.Nucleic acids research · 2005Article
- Mitochondrial membrane potential: a novel biomarker of oxidative environmental stress.Environmental health perspectives · 2002Article
- Abortive base-excision repair of radiation-induced clustered DNA lesions in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper we review recent aspects of the measurement of oxidized DNA bases, currently a matter of debate. There has long been an interest in the determination of the level of oxidized bases in cellular DNA under both normal and oxidative stress conditions. In this respect, the situation is confusing because variations that may be as large as two orders of magnitude have been reported for the yield of the formation of 8-oxo-7,8-dihydroguanine (8-oxoGua) in similar DNA samples. However, recent findings clearly show that application of several assays like gas chromatography-mass spectrometry (GC-MS) and -32P--postlabeling may lead to a significant overestimation of the level of oxidized bases in cellular DNA. In particular, the silylation step, which is required to make the samples volatile for the GC-MS analysis, has been shown to induce oxidation of normal bases at the level of about one oxidized base per 10(4) normal bases. This has been found to be a general process that applies in particular to 8-oxoGua, 8-oxo-7, 8-dihydroadenine,5-hydroxycytosine, 5-(hydroxymethyl)uracil, and 5-formyluracil. Interestingly, prepurification of the oxidized bases from DNA hydrolysate prior to the derivatization reaction prevents artefactual oxidation. Under these conditions, the level of oxidized bases measured by GC-MS is similar to that obtained by HPLC associated with electrochemical detection (HPLC-EC). It should be added that the level of 8-oxo-7,8-dihydro-2;-deoxyguanosine in control cellular DNA has been found to be about fivefold lower than in earlier HPLC-EC measurements by using appropriate conditions of extraction and enzymatic digestion of DNA. Similar conclusions were reached by measuring formamidopyrimidine-DNA glycosylase sensitive sites as revealed by the single cell gel electrophoresis (comet) assay.
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