ArticlePlant molecular biology1997
Regulated expression of plant tRNA genes by the prokaryotic tet and lac repressors.
Article in Plant molecular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 17 citations in OpenAlex.
- Engineering DNA recognition and allosteric response properties of TetR family proteins by using a module-swapping strategy.Nucleic acids research · 2019Article
- Engineering repressors with coevolutionary cues facilitates toggle switches with a master reset.Nucleic acids research · 2019Article
- The TetR family of transcriptional repressors.Microbiology and molecular biology reviews : MMBR · 2005Review
- Suppression of nonsense mutations in cell culture and mice by multimerized suppressor tRNA genes.Molecular and cellular biology · 2000Article
- Tetracycline-regulated suppression of amber codons in mammalian cells.Molecular and cellular biology · 1998Article
- Nonsense and missense translational suppression in plant cells mediated by tRNA(Lys).Plant molecular biology · 1998Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The prokaryotic tet operator (tetO) sequence was inserted at positions upstream and downstream of sequences encoding the Arabidopsis thaliana tRNA(Lys)AUC or tRNA(Trp)AUC suppressor tRNAs, and tRNA expression in carrot protoplasts was measured by translational suppression of a nonsense codon in a luciferase reporter gene. Regulation of tRNA expression by the tetracycline repressor (tetR) occurred from genes with the tetO inserted at position -1 (for the tRNA(Trp)AUC gene), or at positions -2, -6 and -10 (for the tRNA(Lys)AUC gene), and repression reached 90%. The inducer tetracycline (Tc) restored tRNA expression. Similarly, carrot protoplasts transfected with human tRNA(Ser)AUC genes containing the lac operator (lacO) in their 5'-flanking sequence with or without the lac repressor (lacI) gene, conditionally expressed tRNAs which suppressed the luciferase reporter. Up to 30-fold repression occurred by the lactose repressor when lacO was located at position -1 of the tRNA(Ser)AUC coding sequence. In the presence of the inducer isopropyl-beta-thiogalactoside (IPTG), repression was relieved. These results demonstrate that sequences flanking tRNA genes can strongly influence tRNA expression in plants, and in a conditional fashion when bound by inducible proteins.
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