ArticleMolecular biology of the cell1997
Segregation of two spectrin isoforms: polarized membrane-binding sites direct polarized membrane skeleton assembly.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 76 citations in OpenAlex.
- Competition between myosin II and βeLife · 2023Article
- Spectrin regulates cell contractility through production and maintenance of actin bundles in the Caenorhabditis elegans spermatheca.Molecular biology of the cell · 2018Article
- A fixation method to preserve cultured cell cytonemes facilitates mechanistic interrogation of morphogen transport.Development (Cambridge, England) · 2017Article
- Specification of regional intestinal stem cell identity during Drosophila metamorphosis.Development (Cambridge, England) · 2014Article
- Genetic studies of spectrin in the larval fat body of Drosophila melanogaster: evidence for a novel lipid uptake apparatus.Genetics · 2013Article
- The Drosophila Anion Exchanger (DAE) lacks a detectable interaction with the spectrin cytoskeleton.Journal of negative results in biomedicine · 2010Article
- The assembly and maintenance of epithelial junctions in C. elegans.Frontiers in bioscience (Landmark edition) · 2009Review
- Varicose: a MAGUK required for the maturation and function of Drosophila septate junctions.BMC developmental biology · 2008Article
- Mating induces an immune response and developmental switch in the Drosophila oviduct.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton.The Journal of biological chemistry · 2008Article
- New synaptic bouton formation is disrupted by misregulation of microtubule stability in aPKC mutants.Neuron · 2004Article
- Cytoplasmic dynein-dynactin complex is required for spermatid growth but not axoneme assembly in Drosophila.Molecular biology of the cell · 2004Article
- Neuroglian, Gliotactin, and the Na+/K+ ATPase are essential for septate junction function in Drosophila.The Journal of cell biology · 2003Article
- Crumbs interacts with moesin and beta(Heavy)-spectrin in the apical membrane skeleton of Drosophila.The Journal of cell biology · 2002Article
- Alpha spectrin is essential for morphogenesis and body wall muscle formation in Caenorhabditis elegans.The Journal of cell biology · 2002Article
- Drosophila alpha- and beta-spectrin mutations disrupt presynaptic neurotransmitter release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Neuralized functions cell autonomously to regulate Drosophila sense organ development.The EMBO journal · 2000Article
- The L1-type cell adhesion molecule neuroglian influences the stability of neural ankyrin in the Drosophila embryo but not its axonal localization.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Caenorhabditis elegans beta-G spectrin is dispensable for establishment of epithelial polarity, but essential for muscular and neuronal function.The Journal of cell biology · 2000Article
- Drosophila beta spectrin functions independently of alpha spectrin to polarize the Na,K ATPase in epithelial cells.The Journal of cell biology · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Spectrin isoforms are often segregated within specialized plasma membrane subdomains where they are thought to contribute to the development of cell surface polarity. It was previously shown that ankyrin and beta spectrin are recruited to sites of cell-cell contact in Drosophila S2 cells expressing the homophilic adhesion molecule neuroglian. Here, we show that neuroglian has no apparent effect on a second spectrin isoform (alpha beta H), which is constitutively associated with the plasma membrane in S2 cells. Another membrane marker, the Na,K-ATPase, codistributes with ankyrin and alpha beta spectrin at sites of neuroglian-mediated contact. The distributions of these markers in epithelial cells in vivo are consistent with the order of events observed in S2 cells. Neuroglian, ankyrin, alpha beta spectrin, and the Na,K-ATPase colocalize at the lateral domain of salivary gland cells. In contrast, alpha beta H spectrin is sorted to the apical domain of salivary gland and somatic follicle cells. Thus, the two spectrin isoforms respond independently to positional cues at the cell surface: in one case an apically sorted receptor and in the other case a locally activated cell-cell adhesion molecule. The results support a model in which the membrane skeleton behaves as a transducer of positional information within cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.