ArticleMolecular biology of the cell1997
Characterization of the Golgi complex cleared of proteins in transit and examination of calcium uptake activities.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 110 citations in OpenAlex.
- The evolution of organellar calcium mapping technologies.Cell calcium · 2022Review
- GOPC facilitates the sorting of syndecan-1 in polarized epithelial cells.Molecular biology of the cell · 2022Article
- Comparison of the Cisterna Maturation-Progression Model with the Kiss-and-Run Model of Intra-Golgi Transport: Role of Cisternal Pores and Cargo Domains.International journal of molecular sciences · 2022Article
- Models of Intracellular Transport: Pros and Cons.Frontiers in cell and developmental biology · 2019Article
- A putative calcium-ATPase of the secretory pathway family may regulate calcium/manganese levels in the Golgi apparatus of Entamoeba histolytica.Parasitology research · 2018Article
- c-Src regulates cargo transit via the Golgi in pancreatic acinar cells.Scientific reports · 2018Article
- A Single Sphingomyelin Species Promotes Exosomal Release of Endoglin into the Maternal Circulation in Preeclampsia.Scientific reports · 2017Article
- The Golgi apparatus is a functionally distinct Ca2+ store regulated by the PKA and Epac branches of the β1-adrenergic signaling pathway.Science signaling · 2015Article
- GM130 Regulates Golgi-Derived Spindle Assembly by Activating TPX2 and Capturing Microtubules.Cell · 2015Article
- Nutrient transport in the mammary gland: calcium, trace minerals and water soluble vitamins.Journal of mammary gland biology and neoplasia · 2014Review
- Morpho-functional architecture of the Golgi complex of neuroendocrine cells.Frontiers in endocrinology · 2013Article
- Progestin modulates the lipid profile and sensitivity of breast cancer cells to docetaxel.Molecular and cellular endocrinology · 2012Article
- The KDEL receptor couples to Gαq/11 to activate Src kinases and regulate transport through the Golgi.The EMBO journal · 2012Article
- Intracellular organelles in the saga of Ca2+ homeostasis: different molecules for different purposes?Cellular and molecular life sciences : CMLS · 2012Review
- The role of the Golgi-resident SPCA Ca²⁺/Mn²⁺ pump in ionic homeostasis and neural function.Neurochemical research · 2012Review
- Proteomic dissection of the Arabidopsis Golgi and trans-Golgi network.Frontiers in plant science · 2012Article
- AlphaB-crystallin: a Golgi-associated membrane protein in the developing ocular lens.Investigative ophthalmology & visual science · 2009Article
- Hydrophobic profiles of the tail anchors in SLMAP dictate subcellular targeting.BMC cell biology · 2009Article
- Eps15 mediates vesicle trafficking from the trans-Golgi network via an interaction with the clathrin adaptor AP-1.Molecular biology of the cell · 2008Article
- Trypanosoma brucei ARF1 plays a central role in endocytosis and golgi-lysosome trafficking.Molecular biology of the cell · 2007Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
To characterize endogenous molecules and activities of the Golgi complex, proteins in transit were > 99% cleared from rat hepatocytes by using cycloheximide (CHX) treatment. The loss of proteins in transit resulted in condensation of the Golgi cisternae and stacks. Isolation of a stacked Golgi fraction is equally efficient with or without proteins in transit [control (CTL SGF1) and cycloheximide (CHX SGF1)]. Electron microscopy and morphometric analysis showed that > 90% of the elements could be positively identified as Golgi stacks or cisternae. Biochemical analysis showed that the cis-, medial-, trans-, and TGN Golgi markers were enriched over the postnuclear supernatant 200- to 400-fold with and 400- to 700-fold without proteins in transit. To provide information on a mechanism for import of calcium required at the later stages of the secretory pathway, calcium uptake into CTL SGF1 and CHX SGF1 was examined. All calcium uptake into CTL SGF1 was dependent on a thapsigargin-resistant pump not resident to the Golgi complex and a thapsigargin-sensitive pump resident to the Golgi. Experiments using CHX SGF1 showed that the thapsigargin-resistant activity was a plasma membrane calcium ATPase isoform in transit to the plasma membrane and the thapsigargin-sensitive pump was a sarcoplasmic/endoplasmic reticulum calcium ATPase isoform. In vivo both of these calcium ATPases function to maintain millimolar levels of calcium within the Golgi lumen.
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