ArticleMolecular and cellular biology1997
Amino termini of histones H3 and H4 are required for a1-alpha2 repression in yeast.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 49 citations in OpenAlex.
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- The Cyc8-Tup1 complex inhibits transcription primarily by masking the activation domain of the recruiting protein.Genes & development · 2011Article
- Shields up: the Tup1-Cyc8 repressor complex blocks coactivator recruitment.Genes & development · 2011Article
- Spreading of a corepressor linked to action of long-range repressor hairy.Molecular and cellular biology · 2008Article
- Identification of novel activation mechanisms for FLO11 regulation in Saccharomyces cerevisiae.Genetics · 2008Article
- Activator Gcn4p and Cyc8p/Tup1p are interdependent for promoter occupancy at ARG1 in vivo.Molecular and cellular biology · 2005Article
- The histone fold domain of Cse4 is sufficient for CEN targeting and propagation of active centromeres in budding yeast.Eukaryotic cell · 2004Article
- Ssn6-Tup1 requires the ISW2 complex to position nucleosomes in Saccharomyces cerevisiae.The EMBO journal · 2004Article
- Chromatin rearrangements in the prnD-prnB bidirectional promoter: dependence on transcription factors.Eukaryotic cell · 2004Article
- Functional dissection of the global repressor Tup1 in yeast: dominant role of the C-terminal repression domain.Genetics · 2002Article
- Histone-dependent association of Tup1-Ssn6 with repressed genes in vivo.Molecular and cellular biology · 2002Article
- A two-hybrid system for transactivator bait proteins.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Stability of a human SWI-SNF remodeled nucleosomal array.Molecular and cellular biology · 2001Article
- Critical role for the histone H4 N terminus in nucleosome remodeling by ISWI.Molecular and cellular biology · 2001Article
- Srb7p is a physical and physiological target of Tup1p.The EMBO journal · 2000Article
- A new screen for protein interactions reveals that the Saccharomyces cerevisiae high mobility group proteins Nhp6A/B are involved in the regulation of the GAL1 promoter.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Tup1p represses Mcm1p transcriptional activation and chromatin remodeling of an a-cell-specific gene.The EMBO journal · 2000Article
- Analysis of Groucho-histone interactions suggests mechanistic similarities between Groucho- and Tup1-mediated repression.Nucleic acids research · 2000Article
- Genetic analysis of the role of Pol II holoenzyme components in repression by the Cyc8-Tup1 corepressor in yeast.Genetics · 2000Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
The Saccharomyces cerevisiae alpha2 repressor controls two classes of cell-type-specific genes in yeast through association with different partners. alpha2-Mcm1 complexes repress a cell-specific gene expression in haploid alpha cells and diploid a/alpha cells, while a1-alpha2 complexes repress haploid-specific genes in diploid cells. In both cases, repression is mediated through Ssn6-Tu1 corepressor complexes that are recruited via direct interactions with alpha2. We have previously shown that nucleosomes are positioned adjacent to the alpha2-Mcm1 operator under conditions of repression and that Tupl interacts directly with histones H3 and H4. Here, we examine the role of chromatin in a1-alpha2 repression to determine if chromatin is a general feature of repression by Ssn6-Tup1. We find that mutations in the amino terminus of histone H4 cause a 4- to 11-fold derepression of a reporter gene under a1-alpha2 control, while truncation of the H3 amino terminus has a more modest (3-fold or less) effect. Strikingly, combination of the H3 truncation with an H4 mutation causes a 40-fold decrease in repression, clearly indicating a central role for these histones in a1-alpha2-mediated repression. However, in contrast to the ordered positioning of nucleosomes adjacent to the alpha2-Mcm1 operator, nucleosomes are not positioned adjacent to the a1-alpha2 operator in diploid cells. Our data indicate that chromatin is important to Ssn6-Tup1-mediated repression but that the degrees of chromatin organization directed by these proteins differ at different promoters.
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