ArticleMolecular and cellular biology1997
A complex intronic splicing enhancer from the c-src pre-mRNA activates inclusion of a heterologous exon.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
77 citing papers in PubMed, 133 citations in OpenAlex.
- Spliceosome malfunction causes neurodevelopmental disorders with overlapping features.The Journal of clinical investigation · 2024Article
- Splicing regulation of GFPT1 muscle-specific isoform and its roles in glucose metabolisms and neuromuscular junction.iScience · 2023Article
- The RNA-binding proteins hnRNP H and F regulate splicing of a MYC-dependent HRAS exon in prostate cancer cells.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Exon definitive regions forMolecular therapy. Nucleic acids · 2023Article
- Position-dependent effects of RNA-binding proteins in the context of co-transcriptional splicing.NPJ systems biology and applications · 2023Article
- Liver RBFOX2 regulates cholesterol homeostasis via Scarb1 alternative splicing in mice.Nature metabolism · 2022Article
- Identification and Characterization of a Minimal Functional Splicing Regulatory Protein, PTBP1.Biochemistry · 2020Article
- Protein 4.1R Exon 16 3' Splice Site Activation Requires Coordination among TIA1, Pcbp1, and RBM39 during Terminal Erythropoiesis.Molecular and cellular biology · 2017Article
- Multiple determinants of splicing repression activity in the polypyrimidine tract binding proteins, PTBP1 and PTBP2.RNA (New York, N.Y.) · 2016Article
- Alternative Splicing Shapes the Phenotype of a Mutation in BBS8 To Cause Nonsyndromic Retinitis Pigmentosa.Molecular and cellular biology · 2015Article
- Stem-loop 4 of U1 snRNA is essential for splicing and interacts with the U2 snRNP-specific SF3A1 protein during spliceosome assembly.Genes & development · 2014Article
- Identification of functional, short-lived isoform of linker for activation of T cells (LAT).Genes and immunity · 2014Article
- Rbfox3 controls the biogenesis of a subset of microRNAs.Nature structural & molecular biology · 2014Article
- The RNA-binding protein Rbfox2: an essential regulator of EMT-driven alternative splicing and a mediator of cellular invasion.Oncogene · 2014Article
- Quaking and PTB control overlapping splicing regulatory networks during muscle cell differentiation.RNA (New York, N.Y.) · 2013Article
- Combinatorial mutagenesis of MBNL1 zinc fingers elucidates distinct classes of regulatory events.Molecular and cellular biology · 2012Article
- HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing.Nucleic acids research · 2012Article
- Rare inherited A2BP1 deletion in a proband with autism and developmental hemiparesis.American journal of medical genetics. Part A · 2012Article
- A G-rich element forms a G-quadruplex and regulates BACE1 mRNA alternative splicing.Journal of neurochemistry · 2012Article
- The splicing regulator Rbfox2 is required for both cerebellar development and mature motor function.Genes & development · 2012Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The mouse c-src gene contains a short neuron-specific exon, N1. To characterize the sequences that regulate N1 splicing, we used a heterologous gene, derived from the human beta-globin gene, containing a short internal exon that is usually skipped by the splicing machinery. Various fragments from the src gene were inserted into the globin substrate to measure their effects on the splicing of the test exon. These clones were transiently expressed in neuronal and nonneuronal cell lines, and the level of exon inclusion was measured by primer extension. Several sequences from the N1 exon region induced the splicing of the heterologous exon. The most powerful effect was seen with a sequence from the intron downstream of the N1 exon. This sequence acted as a strong splicing enhancer, activating splicing of the test exon when placed in the intron downstream. The enhancer was strongest in neuronal LA-N-5 cells but also activated splicing in nonneuronal HEK293 cells. Deletion and linker scanning mutagenesis indicate that the enhancer is made up of multiple smaller elements that must act in combination. One of these elements was identified as the sequence UGCAUG. Three copies of this element can strongly activate splicing of the test exon in LA-N-5 neuroblastoma cells. These component elements of the src splicing enhancer are also apparently involved in the splicing of other short cassette exons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.