ArticleMolecular and cellular biology1997
Constitutive expression, not a particular primary sequence, is the important feature of the H3 replacement variant hv2 in Tetrahymena thermophila.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Transcriptome analysis of the binucleate ciliateMolecular biology of the cell · 2023Article
- Exploring the Histone Acetylation Cycle in the Protozoan ModelFrontiers in cell and developmental biology · 2020Review
- Review
- Epigenetics of ciliates.Cold Spring Harbor perspectives in biology · 2013Review
- Gene expression studies for the analysis of domoic acid production in the marine diatom Pseudo-nitzschia multiseries.BMC molecular biology · 2013Article
- Impaired replication elongation in Tetrahymena mutants deficient in histone H3 Lys 27 monomethylation.Genes & development · 2013Article
- Quantitative proteomics reveals that the specific methyltransferases Txr1p and Ezl2p differentially affect the mono-, di- and trimethylation states of histone H3 lysine 27 (H3K27).Molecular & cellular proteomics : MCP · 2013Article
- A developmentally regulated gene, ASI2, is required for endocycling in the macronuclear anlagen of Tetrahymena.Eukaryotic cell · 2010Article
- Role of apoptosis-inducing factor (AIF) in programmed nuclear death during conjugation in Tetrahymena thermophila.BMC cell biology · 2010Article
- Dynamic nuclear reorganization during genome remodeling of Tetrahymena.Biochimica et biophysica acta · 2008Review
- Deposition and function of histone H3 variants in Tetrahymena thermophila.Molecular and cellular biology · 2006Article
- Identification of a dehydration and ABA-responsive promoter regulon and isolation of corresponding DNA binding proteins for the group 4 LEA gene CpC2 from C. plantagineum.Plant molecular biology · 2006Article
- Deletion of the Tetrahymena thermophila rDNA replication fork barrier region disrupts macronuclear rDNA excision and creates a fragile site in the micronuclear genome.Nucleic acids research · 2006Article
- Elimination of foreign DNA during somatic differentiation in Tetrahymena thermophila shows position effect and is dosage dependent.Eukaryotic cell · 2005Article
- A new family of "H3L-like" histone genes.Journal of molecular evolution · 2004Article
- Dynamic methylation of histone H3 at lysine 4 in transcriptional regulation by the androgen receptor.Nucleic acids research · 2003Article
- Chromatin assembly at kinetochores is uncoupled from DNA replication.The Journal of cell biology · 2000Article
- Histone H2A.Z has a conserved function that is distinct from that of the major H2A sequence variants.Nucleic acids research · 2000Article
- A nonessential HP1-like protein affects starvation-induced assembly of condensed chromatin and gene expression in macronuclei of Tetrahymena thermophila.Molecular and cellular biology · 1999Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Although quantitatively minor replication-independent (replacement) histone variants have been found in a wide variety of organisms, their functions remain unknown. Like the H3.3 replacement variants of vertebrates, hv2, an H3 variant in the ciliated protozoan Tetrahymena thermophila, is synthesized and deposited in nuclei of nongrowing cells. Although hv2 is clearly an H3.3-like replacement variant by its expression, sequence analysis indicates that it evolved independently of the H3.3 variants of multicellular eukaryotes. This suggested that it is the constitutive synthesis, not the particular protein sequence, of these variants that is important in the function of H3 replacement variants. Here, we demonstrate that the gene (HHT3) encoding hv2 or either gene (HHT1 or HHT2) encoding the abundant major H3 can be completely knocked out in Tetrahymena. Surprisingly, when cells lacking hv2 are starved, a major histone H3 mRNA transcribed by the HHT2 gene, which is synthesized little, if at all, in wild-type nongrowing cells, is easily detectable. Both HHT2 and HHT3 knockout strains show no obvious defect during vegetative growth. In addition, a mutant with the double knockout of HHT1 and HHT3 is viable while the HHT2 HHT3 double-knockout mutant is not. These results argue strongly that cells require a constitutively expressed H3 gene but that the particular sequence being expressed is not critical.
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