ArticleMolecular and cellular biology1997
Role of UME6 in transcriptional regulation of a DNA repair gene in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Global alterations of the transcriptional landscape during yeast growth and development in the absence of Ume6-dependent chromatin modification.Molecular genetics and genomics : MGG · 2015Article
- Mcm2 phosphorylation and the response to replicative stress.BMC genetics · 2012Article
- The histone H3K36 demethylase Rph1/KDM4 regulates the expression of the photoreactivation gene PHR1.Nucleic acids research · 2011Article
- Unraveling transcriptional regulatory programs by integrative analysis of microarray and transcription factor binding data.Bioinformatics (Oxford, England) · 2008Article
- UME6, a novel filament-specific regulator of Candida albicans hyphal extension and virulence.Molecular biology of the cell · 2008Article
- Genome-wide analysis of sterol-lipid storage and trafficking in Saccharomyces cerevisiae.Eukaryotic cell · 2008Article
- The Ume6 regulon coordinates metabolic and meiotic gene expression in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Widespread collaboration of Isw2 and Sin3-Rpd3 chromatin remodeling complexes in transcriptional repression.Molecular and cellular biology · 2001Article
- Identification of the Sin3-binding site in Ume6 defines a two-step process for conversion of Ume6 from a transcriptional repressor to an activator in yeast.Molecular and cellular biology · 2001Article
- Combinatorial regulation of phospholipid biosynthetic gene expression by the UME6, SIN3 and RPD3 genes.Nucleic acids research · 2000Article
- Saccharomyces cerevisiae Ras/cAMP pathway controls post-diauxic shift element-dependent transcription through the zinc finger protein Gis1.The EMBO journal · 2000Article
- RPH1 and GIS1 are damage-responsive repressors of PHR1.Molecular and cellular biology · 1999Article
- Global response of Saccharomyces cerevisiae to an alkylating agent.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae UV radiation and a variety of chemical DNA-damaging agents induce the transcription of specific genes, including several involved in DNA repair. One of the best characterized of these genes is PHR1, which encodes the apoenzyme for DNA photolyase. Basal-level and damage-induced expression of PHR1 require an upstream activation sequence, UAS(PHR1), which has homology with DRC elements found upstream of at least 19 other DNA repair and DNA metabolism genes in yeast. Here we report the identification of the UME6 gene of S. cerevisiae as a regulator of UAS(PHR1) activity. Multiple copies of UME6 stimulate expression from UAS(PHR1) and the intact PHR1 gene. Surprisingly, the effect of deletion of UME6 is growth phase dependent. In wild-type cells PHR1 is induced in late exponential phase, concomitant with the initiation of glycogen accumulation that precedes the diauxic shift. Deletion of UME6 abolishes this induction, decreases the steady-state concentration of photolyase molecules and PHR1 mRNA, and increases the UV sensitivity of a rad2 mutant. Despite the fact that UAS(PHR1) does not contain the URS1 sequence, which has been previously implicated in UME6-mediated transcriptional regulation, we find that Ume6p binds to UAS(PHR1) with an affinity and a specificity similar to those seen for a URS1 site. Similar binding is also seen for DRC elements from RAD2, RAD7, and RAD53, suggesting that UME6 contributes to the regulated expression of a subset of damage-responsive genes in yeast.
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