ArticleJournal of virology1997
Mutational analysis of human immunodeficiency virus type 1 (HIV-1) accessory genes: requirement of a site in the nef gene for HIV-1 replication in activated CD4+ T cells in vitro and in vivo.
Article in Journal of virology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 48 citations in OpenAlex.
- Dynamics and mechanisms of clonal expansion of HIV-1-infected cells in a humanized mouse model.Scientific reports · 2017Article
- A high excision potential of TALENs for integrated DNA of HIV-based lentiviral vector.PloS one · 2015Article
- HIV-1 Vpr accelerates viral replication during acute infection by exploitation of proliferating CD4+ T cells in vivo.PLoS pathogens · 2013Article
- Vpu augments the initial burst phase of HIV-1 propagation and downregulates BST2 and CD4 in humanized mice.Journal of virology · 2012Article
- Remarkable lethal G-to-A mutations in vif-proficient HIV-1 provirus by individual APOBEC3 proteins in humanized mice.Journal of virology · 2010Article
- Uncoating of human immunodeficiency virus type 1 requires prolyl isomerase Pin1.The Journal of biological chemistry · 2010Article
- The utilization of humanized mouse models for the study of human retroviral infections.Retrovirology · 2009Review
- Primary human immunodeficiency virus type 1 nef alleles show major differences in pathogenicity in transgenic mice.Journal of virology · 2007Article
- Wild-type levels of nuclear localization and human immunodeficiency virus type 1 replication in the absence of the central DNA flap.Journal of virology · 2002Article
- The pathogenicity of human immunodeficiency virus (HIV) type 1 Nef in CD4C/HIV transgenic mice is abolished by mutation of its SH3-binding domain, and disease development is delayed in the absence of Hck.Journal of virology · 2001Article
- Critical contribution of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) to apoptosis of human CD4+ T cells in HIV-1-infected hu-PBL-NOD-SCID mice.The Journal of experimental medicine · 2001Article
- Inhibitory mechanism of the CXCR4 antagonist T22 against human immunodeficiency virus type 1 infection.Journal of virology · 1999Article
- Determinant in human immunodeficiency virus type 1 for efficient replication under cytokine-induced CD4(+) T-helper 1 (Th1)- and Th2-type conditions.Journal of virology · 1999Article
- Regions of human immunodeficiency virus type 1 nef required for function in vivo.Journal of virology · 1998Article
Corrections and comments
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human immunodeficiency virus type 1 (HIV-1) accessory genes including nef, vif, and vpr are important factors that determine the replication and pathogenesis of HIV-1. The state of activation is also important for the replication of HIV-1. We evaluated the properties of nef-, vif-, and vpr-minus macrophage-tropic HIV-1(JR) CSF in primary CD4+ Th1- or Th2-like cell cultures which had been activated through CD3 molecules in the presence of interleukin-2 (IL-2) and IL-12 (Th1-like culture) or IL-4 (Th2-like culture), respectively. In activated Th1- or Th2-like cultures, replication of nef-minus HIV-1(JR-CSF) was markedly lower than that of wild-type HIV-1. Subsequent analysis by site-directed mutagenesis showed that (i) the presence of an acidic amino acid-rich domain (amino acid residues 72 to 75) in the Nef protein was critical for the enhancement of viral DNA synthesis, resulting in increased virus growth rate, and (ii) prolines that form part of Src homology 3 binding domain were not essential for viral replication. We also confirmed the importance of sites by using an HIV-1-infected animal model, the hu-PBL-SCID mouse system, representing HIV-1 replication and pathogenesis in activated CD4+ T cells in vivo. These results indicate that Nef accelerates viral replication in activated CD4+ T cells.
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