ArticlePlant physiology1997
Epigenetic transcriptional silencing and 5-azacytidine-mediated reactivation of a complex transgene in rice.
Article in Plant physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 126 citations in OpenAlex.
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- Stochastic Variation in DNA Methylation Modulates Nucleosome Occupancy and Alternative Splicing inPlants (Basel, Switzerland) · 2022Article
- Epigenetic Variation Induced by Gamma Rays, DNA Methyltransferase Inhibitors, and Their Combination in Rice.Plants (Basel, Switzerland) · 2020Article
- Progressive 35S promoter methylation increases rapidly during vegetative development in transgenic Nicotiana attenuata plants.BMC plant biology · 2013Article
- Epigenetic memory of DNAi associated with cytosine methylation and histone modification in fern.Plant signaling & behavior · 2012Article
- Reliable transient transformation of intact maize leaf cells for functional genomics and experimental study.Plant physiology · 2012Article
- Ubiquitin promoter-terminator cassette promotes genetically stable expression of the taste-modifying protein miraculin in transgenic lettuce.Plant cell reports · 2011Article
- Real-time PCR for the detection of precise transgene copy number in durum wheat.Cellular & molecular biology letters · 2011Article
- Article
- Genetic transformation of Sorghum bicolor.Physiology and molecular biology of plants : an international journal of functional plant biology · 2009Article
- RNA silencing mediated by direct repeats in maize: a potential tool for functional genomics.Molecular biotechnology · 2009Article
- Epigenetic inheritance in rice plants.Annals of botany · 2007Article
- Agrobacterium-mediated transformation of the dwarf pomegranate (Punica granatum L. var. nana).Plant cell reports · 2007Article
- Stability of transgene integration and expression in subsequent generations of doubled haploid oilseed rape transformed with chitinase and beta-1,3-glucanase genes in a double-gene construct.Plant cell reports · 2006Article
- Organization, not duplication, triggers silencing in a complex transgene locus in rice.Plant molecular biology · 2005Article
- A two-edged role for the transposable element Kiddo in the rice ubiquitin2 promoter.The Plant cell · 2005Article
- Matrix attachment regions increase the efficiency and stability of RNA-mediated resistance to tomato spotted wilt virus in transgenic tobacco.Transgenic research · 2005Article
- Article
- Methylation of the exon/intron region in the Ubi1 promoter complex correlates with transgene silencing in barley.Plant molecular biology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite a growing number of reports indicating non-Mendelian inheritance of transgene expression in monocots, no detailed description of the structure and stability of the transgene exists for transformants generated by direct DNA-transfer techniques, making the cause for these observations difficult to determine. In this paper we describe the complex organization of Btt cryIIIA and bar transgenes in rice (Oryza sativa L.) that displayed aberrant segregation in R1 progeny. Silencing rather than rearrangement of the bar gene was implicated because the herbicide-sensitive R1 plants had a DNA hybridization profile identical to that of the resistant R0 parent and R1 siblings. Genomic DNA analysis revealed substantial methylation of the Ubi1/bar sequences in silenced plants and, to a lesser degree, in herbicide-resistant plants, suggesting that the transgene locus was potentiated for silencing. Nuclease protection and nuclear run-on assays confirmed that silencing was due to transcriptional inactivation. Treatment of R2 progeny of silenced plants with 5-azacytidine resulted in demethylation of the Ubi1 promoter and reactivation of bar gene expression, demonstrating a functional relationship for methylation in gene silencing. These findings indicate that methylation-based silencing may be frequent in cereals transformed by direct DNA protocols that insert multiple, often rearranged sequences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.