ArticleProceedings of the National Academy of Sciences of the United States of America1997
Extensive purification of a putative RNA polymerase I holoenzyme from plants that accurately initiates rRNA gene transcription in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 61 citations in OpenAlex.
- DNA-dependent RNA polymerases in plants.The Plant cell · 2023Article
- Reaching for the off switch in nucleolar dominance.The Plant journal : for cell and molecular biology · 2023Article
- Rescue of tomato spotted wilt virus entirely from complementary DNA clones.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Ribosome Biogenesis in Plants: From Functional 45S Ribosomal DNA Organization to Ribosome Assembly Factors.The Plant cell · 2019Review
- Nucleolar Proteome Analysis and Proteasomal Activity Assays Reveal a Link between Nucleolus and 26S Proteasome inFrontiers in plant science · 2017Article
- Arabidopsis Pol II-Dependent in Vitro Transcription System Reveals Role of Chromatin for Light-Inducible rbcS Gene Transcription.Plant physiology · 2016Article
- Chromosome-specific NOR inactivation explains selective rRNA gene silencing and dosage control in Arabidopsis.Genes & development · 2016Article
- Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit.Nucleic acids research · 2015Article
- Multisubunit RNA polymerases IV and V: purveyors of non-coding RNA for plant gene silencing.Nature reviews. Molecular cell biology · 2011Review
- Processing of a dicistronic tRNA-snoRNA precursor: combined analysis in vitro and in vivo reveals alternate pathways and coupling to assembly of snoRNP.Plant physiology · 2009Article
- Subunit compositions of the RNA-silencing enzymes Pol IV and Pol V reveal their origins as specialized forms of RNA polymerase II.Molecular cell · 2009Article
- Multimegabase silencing in nucleolar dominance involves siRNA-directed DNA methylation and specific methylcytosine-binding proteins.Molecular cell · 2008Article
- Modulation of RNA polymerase assembly dynamics in transcriptional regulation.Molecular cell · 2008Article
- Characterization of AtNUC-L1 reveals a central role of nucleolin in nucleolus organization and silencing of AtNUC-L2 gene in Arabidopsis.Molecular biology of the cell · 2007Article
- Mechanisms of genomic rearrangements and gene expression changes in plant polyploids.BioEssays : news and reviews in molecular, cellular and developmental biology · 2006Review
- Organization, differential expression and methylation of rDNA in artificial Solanum allopolyploids.Plant molecular biology · 2004Article
- Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription.Molecular and cellular biology · 2004Article
- Multiple interactions between RNA polymerase I, TIF-IA and TAF(I) subunits regulate preinitiation complex assembly at the ribosomal gene promoter.EMBO reports · 2002Article
- Transcription and tyranny in the nucleolus: the organization, activation, dominance and repression of ribosomal RNA genes.The arabidopsis book · 2002Article
- RNA polymerase I holoenzyme-promoter complexes include an associated CK2-like protein kinase.Plant molecular biology · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
RNA polymerase I (pol I) is a nuclear enzyme whose function is to transcribe the duplicated genes encoding the precursor of the three largest ribosomal RNAs. We report a cell-free system from broccoli (Brassica oleracea) inflorescence that supports promoter-dependent RNA pol I transcription in vitro. The transcription system was purified extensively by DEAE-Sepharose, Biorex 70, Sephacryl S300, and Mono Q chromatography. Activities required for pre-rRNA transcription copurified with the polymerase on all four columns, suggesting their association as a complex. Purified fractions programmed transcription initiation from the in vivo start site and utilized the same core promoter sequences required in vivo. The complex was not dissociated in 800 mM KCl and had a molecular mass of nearly 2 MDa based on gel filtration chromatography. The most highly purified fractions contain approximately 30 polypeptides, two of which were identified immunologically as RNA polymerase subunits. These data suggest that the occurrence of a holoenzyme complex is probably not unique to the pol II system but may be a general feature of eukaryotic nuclear polymerases.
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Registered trials
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