ArticleThe Biochemical journal1997
Involvement of proteasomal subunits zeta and iota in RNA degradation.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 44 citations in OpenAlex.
- Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.Nature communications · 2019Article
- Tomato 26S Proteasome subunit RPT4a regulates ToLCNDV transcription and activates hypersensitive response in tomato.Scientific reports · 2016Article
- Review
- Does proteasome regulate the level of microRNA-1 in cardiomyocytes? Application to anoxia-reoxygenation.Molecular and cellular biochemistry · 2015Article
- Proteomic response of Rhizoctonia solani GD118 suppressed by Paenibacillus kribbensis PS04.World journal of microbiology & biotechnology · 2014Article
- Article
- The 20S proteasome α5 subunit of Arabidopsis thaliana carries an RNase activity and interacts in planta with the lettuce mosaic potyvirus HcPro protein.Molecular plant pathology · 2011Article
- The ubiquitin/26S proteasome system in plant-pathogen interactions: a never-ending hide-and-seek game.Molecular plant pathology · 2010Review
- Assembly of AUF1 with eIF4G-poly(A) binding protein complex suggests a translation function in AU-rich mRNA decay.RNA (New York, N.Y.) · 2006Article
- alpha5 subunit in Trypanosoma brucei proteasome can self-assemble to form a cylinder of four stacked heptamer rings.The Biochemical journal · 1999Article
- Proteasome subunit zeta, a putative ribonuclease, is also found as a free monomer.Molecular biology reports · 1999Article
- Relationships between proteasomes and viral gene products.Molecular biology reports · 1999Review
- Proteasome (prosome) associated endonuclease activity.Molecular biology reports · 1997Review
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have identified two distinct subunits of 20 S proteasomes that are associated with RNase activity. Proteasome subunits zeta and iota, eluted from two-dimensional Western blots, hydrolysed tobacco mosaic virus RNA, whereas none of the other subunits degraded this substrate under the same conditions. Additionally, proteasomes were dissociated by 6 M urea, and subunit zeta, containing the highest RNase activity, was isolated by anion-exchange chromatography and gel filtration. Purified subunit zeta migrated as a single spot on two-dimensional PAGE with a molecular mass of approx. 28 kDa. Addition of anti-(subunit zeta) antibodies led to the co-precipitation of this proteasome subunit and nuclease activity. This is the first evidence that proteasomal alpha-type subunits are associated with an enzymic activity, and our results provide further evidence that proteasomes may be involved in cellular RNA metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.