ArticleNucleic acids research1997
Position effects in mice carrying a lacZ transgene in cis with the beta-globin LCR can be explained by a graded model.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 38 citations in OpenAlex.
- Genetic Animal Models of Cardiovascular Pathologies.Biomedicines · 2025Review
- Comparative analysis of two independent Myh6-Cre transgenic mouse lines.Journal of molecular and cellular cardiology plus · 2024Article
- Relative transgene expression frequencies in homozygous versus hemizygous transgenic mice.Transgenic research · 2013Article
- The SCL +40 enhancer targets the midbrain together with primitive and definitive hematopoiesis and is regulated by SCL and GATA proteins.Molecular and cellular biology · 2007Article
- Artificial chromosome-based transgenes in the study of genome function.Mammalian genome : official journal of the International Mammalian Genome Society · 2006Review
- Transcriptional regulation of the SCL locus: identification of an enhancer that targets the primitive erythroid lineage in vivo.Molecular and cellular biology · 2005Article
- Analysis of cell-specificity and variegation of transgene expression driven by salmon prolactin promoter in stable lines of transgenic rainbow trout.Transgenic research · 2003Article
- Tetracycline-inducible system for regulation of skeletal muscle-specific gene expression in transgenic mice.Transgenic research · 2003Article
- Activation of the beta globin locus by transcription factors and chromatin modifiers.The EMBO journal · 2000Article
- The changing role of cell culture in the generation of transgenic livestock.Cytotechnology · 1999Article
- Enhancement of beta-globin locus control region-mediated transactivation by mitogen-activated protein kinases through stochastic and graded mechanisms.Molecular and cellular biology · 1999Article
- Derepression of human embryonic zeta-globin promoter by a locus-control region sequence.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Stochastic, stage-specific mechanisms account for the variegation of a human globin transgene.Nucleic acids research · 1998Article
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied transgenic mice carrying the lacZ reporter gene linked to the erythroid-specific beta-globin promoter and beta-globin locus control region (LCR). Previously, we had demonstrated that the total level of expression of beta-galactosidase enzyme, which is the product of the lacZ gene, varies widely between different transgenic mice due to position effects at the sites of transgene integration. Here, using the X-gal based in situ assay for beta-galactosidase activity, we found that the percent erythroid cells that expressed the transgene also varied widely between the mice. Moreover, a kinetic analysis showed that the average beta-galactosidase content per expressing cell varied both between samples of different transgenic descent and between erythroid cells within each sample, demonstrating that the variable expression of this lacZ transgene was being controlled in a graded manner. These results suggest that the beta-globin LCR enhancers function through a graded model, which is described, rather than the binary mechanism that has been proposed previously for other enhancers.
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