ArticleBiophysical journal1997
Mechanics of single kinesin molecules measured by optical trapping nanometry.
Article in Biophysical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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Who cites it
96 citing papers in PubMed, 311 citations in OpenAlex.
- Article
- Stall force measurement of the kinesin-3 motor KIF1A using a programmable DNA origami nanospring.eLife · 2026Article
- Active fluctuations of cytoplasmic actomyosin networks facilitate dynein-driven transport.iScience · 2025Article
- An Overview of Contrasting Experimental Results on Dynamics of Kinesin-1 Molecular Motors: Insight into the Underlying Mechanism.Biomolecules · 2025Review
- DNA tensiometer reveals catch-bond detachment kinetics of kinesin-1, -2 and -3.bioRxiv : the preprint server for biology · 2025Article
- Measuring molecular forces inside living cells using magnetic tweezers.Biophysical reviews · 2025Review
- Nonthermal fluctuations accelerate biomolecular motors.Biophysical reviews · 2024Review
- Competition between physical search and a weak-to-strong transition rate-limits kinesin binding times.PLoS computational biology · 2024Article
- ADP release can explain spatially-dependent kinesin binding times.bioRxiv : the preprint server for biology · 2023Article
- Versatile properties of dynein molecules underlying regulation in flagellar oscillation.Scientific reports · 2023Article
- Direct Measurement of Kinetic Force Generated by Mycoplasma.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Axonal transport during injury on a theoretical axon.Frontiers in cellular neuroscience · 2023Article
- Force generation of KIF1C is impaired by pathogenic mutations.Current biology : CB · 2022Article
- Kinesin Motors in the Filamentous Basidiomycetes in Light of theJournal of fungi (Basel, Switzerland) · 2022Review
- Autoregulation and dual stepping mode of MYA2, an Arabidopsis myosin XI responsible for cytoplasmic streaming.Scientific reports · 2022Article
- Microtubule Dumbbells to Assess the Effect of Force Geometry on Single Kinesin Motors.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Force and Stepwise Movements of Gliding Motility in Human Pathogenic BacteriumFrontiers in microbiology · 2021Article
- Optical tweezers in single-molecule biophysics.Nature reviews. Methods primers · 2021Article
- Effective behavior of cooperative and nonidentical molecular motors.Research in the mathematical sciences · 2020Article
- Modelling cytoskeletal transport by clusters of non-processive molecular motors with limited binding sites.Royal Society open science · 2020Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analyzed the mechanics of individual kinesin molecules by optical trapping nanometry. A kinesin molecule was adsorbed onto a latex bead, which was captured by an optical trap and brought into contact with an axoneme that was bound to a glass surface. The displacement of kinesin during force generation was determined by measuring the position of the beads with nanometer accuracy. As the displacement of kinesin was attenuated because of the compliance of the kinesin-to-bead and kinesin-to-microtubule linkages, the compliance was monitored during force generation and was used to correct the displacement of kinesin. Thus the velocity and the unitary steps could be obtained accurately over a wide force range. The force-velocity curves were linear from 0 to a maximum force at 10 microM and 1 mM ATP, and the maximum force was approximately 7 pN, which is larger by approximately 30% than values previously reported. Kinesin exhibited forward and occasionally backward stepwise displacements with a size of approximately 8 nm. The histograms of step dwell time show a monotonic decrease with time. Model calculations indicate that each kinesin head steps by 16-nm, whereas kinesin molecule steps by 8-nm.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.