ArticleGenetics1997
The role of Gcr1p in the transcriptional activation of glycolytic genes in yeast Saccharomyces cerevisiae.
Article in Genetics, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 50 citations in OpenAlex.
- Active compensation for changes in TDH3 expression mediated by direct regulators of TDH3 in Saccharomyces cerevisiae.PLoS genetics · 2023Article
- Mutational sources ofeLife · 2021Article
- Identification of a Heat-Inducible Element of Cysteine Desulfurase Gene Promoter inMolecules (Basel, Switzerland) · 2019Article
- Article
- Biochemical characterization of human Ecdysoneless reveals a role in transcriptional regulation.Biological chemistry · 2010Article
- Promoter library designed for fine-tuned gene expression in Pichia pastoris.Nucleic acids research · 2008Article
- A cis-regulatory logic simulator.BMC bioinformatics · 2007Article
- Fine-structure analysis of ribosomal protein gene transcription.Molecular and cellular biology · 2006Article
- Identifying cooperative transcriptional regulations using protein-protein interactions.Nucleic acids research · 2005Article
- Mutations in GCR1 affect SUC2 gene expression in Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2003Article
- Trehalose, glycogen and ethanol metabolism in the gcr1 mutant of Saccharomyces cerevisiae.Folia microbiologica · 2003Article
- The hexokinase 2 protein regulates the expression of the GLK1, HXK1 and HXK2 genes of Saccharomyces cerevisiae.The Biochemical journal · 2001Article
- Understanding the growth phenotype of the yeast gcr1 mutant in terms of global genomic expression patterns.Journal of bacteriology · 2000Article
- In vivo analysis of functional regions within yeast Rap1p.Molecular and cellular biology · 1999Article
- Functional characterization of transcriptional regulatory elements in the upstream region of the yeast GLK1 gene.The Biochemical journal · 1999Article
- Glucose metabolism in gcr mutants of Saccharomyces cerevisiae.Journal of bacteriology · 1999Article
- Multiple domains of repressor activator protein 1 contribute to facilitated binding of glycolysis regulatory protein 1.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study the interdependence of Gcr1p and Rap1p, we prepared a series of synthetic regulatory sequences that contained various numbers and combinations of CT-boxes (Gcr1p-binding sites) and RPG-boxes (Rap1p-binding sites). The ability of the synthetic oligonucleotides to function as regulatory sequences was tested using an ENO1-lacZ reporter gene. As observed previously, synthetic oligonucleotides containing both CT- and RPG-boxes conferred strong UAS activity. Likewise, a lone CT-box did not show any UAS activity. By contrast, oligonucleotides containing tandem Ct-boxes but no RPG-box conferred strong promoter activity. This UAS activity was not dependent on position or orientation of the oligonucleotides in the 5' noncoding region. However, it was dependent on both GCR1 and GCR2. These results suggest that the ability of Gcr1p to bind Gcr1p-binding sites in vivo is not absolutely dependent on Rap1p. Eleven independent mutants of GCR1 were isolated that conferred weak UAS activity to a single CT-box. Five mutants has single mutations in Gcr1p's DNA-binding domain and displayed slightly higher affinity for the CT-box. These results support the hypothesis that Gcr1p and Gcr2p play the central role in glycolytic gene expression and that the function of Rap1p is to facilitate the binding of Gcr1p to its target.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.