ArticleGenetics1997
Genetic interactions between a pep7 mutation and the PEP12 and VPS45 genes: evidence for a novel SNARE component in transport between the Saccharomyces cerevisiae Golgi complex and endosome.
Article in Genetics, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Inhibition of Vesicular Transport Influences Fungal Susceptibility to Fluconazole.Antimicrobial agents and chemotherapy · 2019Article
- Identification of putative effectors of the Type IV secretion system from the Wolbachia endosymbiont of Brugia malayi.PloS one · 2018Article
- Functional separation of endosomal fusion factors and the class C core vacuole/endosome tethering (CORVET) complex in endosome biogenesis.The Journal of biological chemistry · 2013Article
- LegC3, an effector protein from Legionella pneumophila, inhibits homotypic yeast vacuole fusion in vivo and in vitro.PloS one · 2013Article
- The role of Munc18-1 and its orthologs in modulation of cortical F-actin in chromaffin cells.Journal of molecular neuroscience : MN · 2012Review
- Regulation of syntaxin1A-munc18 complex for SNARE pairing in HEK293 cells.The Journal of physiology · 2004Article
- The Sec1/Munc18 protein, Vps33p, functions at the endosome and the vacuole of Saccharomyces cerevisiae.Molecular biology of the cell · 2004Article
- deep-orange and carnation define distinct stages in late endosomal biogenesis in Drosophila melanogaster.The Journal of cell biology · 2003Article
- How Tlg2p/syntaxin 16 'snares' Vps45.The EMBO journal · 2002Article
- Vps45p stabilizes the syntaxin homologue Tlg2p and positively regulates SNARE complex formation.The EMBO journal · 2001Article
- Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae.Genetics · 2000Article
- Specific retrieval of the exocytic SNARE Snc1p from early yeast endosomes.Molecular biology of the cell · 2000Article
- A role for Tlg1p in the transport of proteins within the Golgi apparatus of Saccharomyces cerevisiae.Molecular biology of the cell · 1999Article
- The phosphatidylinositol 3-phosphate binding protein Vac1p interacts with a Rab GTPase and a Sec1p homologue to facilitate vesicle-mediated vacuolar protein sorting.Molecular biology of the cell · 1999Article
- Article
- Molecular aspects of the endocytic pathway.The Biochemical journal · 1998Review
- Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The PEP7 gene from Saccharomyces cerevisiae encodes a 59-kD hydrophilic polypeptide that is required for transport of soluble vacuolar hydrolase precursors from the TGN to the endosome. This study presents the results of a high-copy suppression analysis of pep7-20 mutant phenotypes. This analysis demonstrated that both VPS45 and PEP12 are allele-specific high-copy suppressors of pep7-20 mutant phenotypes. Overexpression of VPS45 was able to completely suppress the Zn2+ sensitivity and partially suppress the carboxypeptidase Y deficiency. Overexpression of PEP12 was able to do the same, but to a lesser extent. Vps45p and Pep12p are Sec1p and syntaxin (t-SNARE) homologues, respectively, and are also thought to function in transport between the TGN and endosome. Two additional vacuole pathway SNARE complex homologues, Vps33p (Sec1p) and Pth1p (syntaxin), when overexpressed, were unable to suppress pep7-20 or any other pep7 allele, further supporting the specificity of the interactions of pep7-20 with PEP12 and VPS45. Because several other vesicle docking/fusion reactions take place in the cell without discernible participation of Pep7p homologues, we suggest that Pep7p is a step-specific regulator of docking and/or fusion of TGN-derived transport vesicles onto the endosome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.