ArticleThe Journal of experimental medicine1997
Mutational evidence for control of cell adhesion through integrin diffusion/clustering, independent of ligand binding.
Article in The Journal of experimental medicine, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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Who cites it
42 citing papers in PubMed.
- The Stress-Inducible Protein DRR1 Exerts Distinct Effects on Actin Dynamics.International journal of molecular sciences · 2018Article
- Phosphorylation of the α-chain in the integrin LFA-1 enables β2-chain phosphorylation and α-actinin binding required for cell adhesion.The Journal of biological chemistry · 2018Article
- The membrane-distal regions of integrin α cytoplasmic domains contribute differently to integrin inside-out activation.Scientific reports · 2018Article
- Tetraspanin 8 is a novel regulator of ILK-driven β1 integrin adhesion and signaling in invasive melanoma cells.Oncotarget · 2017Article
- Tetraspanins Function as Regulators of Cellular Signaling.Frontiers in cell and developmental biology · 2017Review
- The dual structural roles of the membrane distal region of the α-integrin cytoplasmic tail during integrin inside-out activation.Journal of cell science · 2015Article
- Aggregatibacter actinomycetemcomitans leukotoxin (LtxA; Leukothera) induces cofilin dephosphorylation and actin depolymerization during killing of malignant monocytes.Microbiology (Reading, England) · 2014Article
- Integrin αII b tail distal of GFFKR participates in inside-out αII b β3 activation.Journal of thrombosis and haemostasis : JTH · 2014Article
- CD151 restricts the α6 integrin diffusion mode.Journal of cell science · 2012Article
- Integrin organization: linking adhesion ligand nanopatterns with altered cell responses.Journal of theoretical biology · 2011Article
- Syndecan-1 couples the insulin-like growth factor-1 receptor to inside-out integrin activation.Journal of cell science · 2010Article
- Noninvasive measurements of integrin microclustering under altered membrane cholesterol levels.Biophysical journal · 2010Article
- R-Ras regulates beta1-integrin trafficking via effects on membrane ruffling and endocytosis.BMC cell biology · 2010Article
- Proapoptotic function of integrin beta(3) in human hepatocellular carcinoma cells.Clinical cancer research : an official journal of the American Association for Cancer Research · 2009Article
- Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell culture.Journal of nanobiotechnology · 2008Article
- Article
- Altered membrane dynamics of quantum dot-conjugated integrins during osteogenic differentiation of human bone marrow derived progenitor cells.Biophysical journal · 2007Article
- Alpha4beta1-dependent adhesion strengthening under mechanical strain is regulated by paxillin association with the alpha4-cytoplasmic domain.The Journal of cell biology · 2005Article
- Elasticity and adhesion force mapping reveals real-time clustering of growth factor receptors and associated changes in local cellular rheological properties.Biophysical journal · 2004Article
- Avidity modulation activates adhesion under flow and requires cooperativity among adhesion receptors.Biophysical journal · 2003Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Previous studies have shown that integrin alpha chain tails make strong positive contributions to integrin-mediated cell adhesion. We now show here that integrin alpha4 tail deletion markedly impairs static cell adhesion by a mechanism that does not involve altered binding of soluble vascular cell adhesion molecule 1 ligand. Instead, truncation of the alpha4 cytoplasmic domain caused a severe deficiency in integrin accumulation into cell surface clusters, as induced by ligand and/ or antibodies. Furthermore, alpha4 tail deletion also significantly decreased the membrane diffusivity of alpha4beta1, as determined by a single particle tracking technique. Notably, low doses of cytochalasin D partially restored the deficiency in cell adhesion seen upon alpha4 tail deletion. Together, these results suggest that alpha4 tail deletion exposes the beta1 cytoplasmic domain, leading to cytoskeletal associations that apparently restrict integrin lateral diffusion and accumulation into clusters, thus causing reduced static cell adhesion. Our demonstration of integrin adhesive activity regulated through receptor diffusion/clustering (rather than through altered ligand binding affinity) may be highly relevant towards the understanding of inside-out signaling mechanisms for beta1 integrins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.