ArticleThe Journal of cell biology1997
Presentation of integrins on leukocyte microvilli: a role for the extracellular domain in determining membrane localization.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 86 citations in OpenAlex.
- Nanoscale imaging of CD47 informs how plasma membrane modifications shape apoptotic cell recognition.Communications biology · 2023Article
- Pre-organized landscape of T cell surface.Frontiers in immunology · 2023Review
- Nanostructured Titanium Implant Surface Facilitating Osseointegration from Protein Adsorption to Osteogenesis: The Example of TiOInternational journal of nanomedicine · 2022Review
- CCR7 signalosomes are preassembled on tips of lymphocyte microvilli in proximity to LFA-1.Biophysical journal · 2021Article
- CD9, a tetraspanin target for cancer therapy?Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Energy landscape differences among integrins establish the framework for understanding activation.The Journal of cell biology · 2018Article
- Ena/VASP proteins regulate activated T-cell trafficking by promoting diapedesis during transendothelial migration.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Three-dimensional localization of T-cell receptors in relation to microvilli using a combination of superresolution microscopies.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- CD47-retargeted oncolytic adenovirus armed with melanoma differentiation-associated gene-7/interleukin-24 suppresses in vivo leukemia cell growth.Oncotarget · 2015Article
- Article
- Ovarian cancer stem cells: are they real and why are they important?Gynecologic oncology · 2014Review
- Regulation of eosinophil trafficking by SWAP-70 and its role in allergic airway inflammation.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Theoretical model for cellular shapes driven by protrusive and adhesive forces.PLoS computational biology · 2011Article
- The transmembrane domains of L-selectin and CD44 regulate receptor cell surface positioning and leukocyte adhesion under flow.The Journal of biological chemistry · 2010Article
- Rolling cell adhesion.Annual review of cell and developmental biology · 2010Review
- Cell adhesion molecule distribution relative to neutrophil surface topography assessed by TIRFM.Biophysical journal · 2009Article
- Molecular accessibility in relation to cell surface topography and compression against a flat substrate.Biophysical journal · 2009Article
- Active site formation, not bond kinetics, limits adhesion rate between human neutrophils and immobilized vascular cell adhesion molecule 1.Biophysical journal · 2009Article
- Cells on the run: shear-regulated integrin activation in leukocyte rolling and arrest on endothelial cells.Current opinion in cell biology · 2008Review
- Fluorescence-topographic NSOM directly visualizes peak-valley polarities of GM1/GM3 rafts in cell membrane fluctuations.Journal of lipid research · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Adhesion of blood leukocytes to the endothelium involves multiple steps including initial attachment (tethering), rolling, and firm arrest. Presentation of adhesion molecules on leukocyte microvilli can substantially enhance tethering. Localization of L-selectin to microvilli and of CD44 to the planar cell body have been shown to depend upon their transmembrane and cytoplasmic domains. We investigated the role of leukocyte integrin transmembrane and cytoplasmic domains in initiating adhesion under flow and in microvillous localization. Integrins alpha4beta7, alphaLbeta2, and alphaMbeta2 were heterologously expressed in K562 cells. alpha4beta7 initiated adhesion under flow and localized to microvilli, whereas beta2 integrins did not initiate adhesion and localized to the cell body. Chimeric integrins were produced by replacing the alpha4beta7 cytoplasmic and/or transmembrane domains with the homologous domains of alphaLbeta2 or alphaMbeta2. Unexpectedly, these chimeras efficiently mediated adhesion to the alpha4beta7 ligand mucosal addressin cell adhesion molecule-1 under flow and localized to microvilli. Therefore, differences between the transmembrane and cytoplasmic domains of alpha4 and beta2 integrins do not account for differences in ability to support attachment under flow or in membrane localization. Integrins alpha4beta1, alpha5beta1, alpha6Abeta1, alphavbeta3, and alphaEbeta7 also localized to microvilli. Transmembrane proteins known or suspected to associate with extracellular domains of microvillous integrins, including tetraspans and CD47, were concentrated on microvilli as well. These findings suggest that interactions between the extracellular domains of integrins and associated proteins could direct the assembly of multimolecular complexes on leukocyte microvilli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.