ArticleThe Journal of cell biology1997
Phosphatidylinositol 3-kinase is required for the formation of constitutive transport vesicles from the TGN.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 82 citations in OpenAlex.
- A Golgi-derived vesicle potentiates PtdIns4P to PtdIns3P conversion for endosome fission.Nature cell biology · 2021Article
- The cellular uptake of angiogenin, an angiogenic and neurotrophic factor is through multiple pathways and largely dynamin independent.PloS one · 2018Article
- Phosphoinositide 3-kinase δ regulates membrane fission of Golgi carriers for selective cytokine secretion.The Journal of cell biology · 2010Article
- Phosphatidylinositol 3-kinase C2alpha is essential for ATP-dependent priming of neurosecretory granule exocytosis.Molecular biology of the cell · 2005Article
- Signal transduction therapeutics: relevance for Alzheimer's disease.Journal of molecular neuroscience : MN · 2004Review
- Lipoarabinomannan-induced cell signaling involves ceramide and mitogen-activated protein kinase.Clinical and diagnostic laboratory immunology · 2002Article
- The trans Golgi network is lost from cells infected with African swine fever virus.Journal of virology · 2001Article
- A new dynamin-like protein, ADL6, is involved in trafficking from the trans-Golgi network to the central vacuole in Arabidopsis.The Plant cell · 2001Article
- Cellular functions of phosphatidylinositol 3-phosphate and FYVE domain proteins.The Biochemical journal · 2001Review
- Effects of staurosporine, U-73122, wortmannin, 4-hydroxynonenal and sodium azide upon the release of secreted beta-amyloid precursor protein from human platelets in response to thrombin stimulation.Molecular and cellular biochemistry · 2001Article
- Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation.The Biochemical journal · 2001Article
- Trafficking of phosphatidylinositol 3-phosphate from the trans-Golgi network to the lumen of the central vacuole in plant cells.The Plant cell · 2001Article
- Phosphoinositide-AP-2 interactions required for targeting to plasma membrane clathrin-coated pits.The Journal of cell biology · 1999Article
- An essential role for the phosphatidylinositol transfer protein in the scission of coatomer-coated vesicles from the trans-Golgi network.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Biogenesis of secretory granules in the trans-Golgi network of neuroendocrine and endocrine cells.Biochimica et biophysica acta · 1998Review
- Sorting and storage during secretory granule biogenesis: looking backward and looking forward.The Biochemical journal · 1998Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
An 85-kD cytosolic complex (p62(cplx)), consisting of a 62-kD phosphoprotein (p62) and a 25-kD GTPase, has been shown to be essential for the cell-free reconstitution of polymeric IgA receptor (pIgA-R)-containing exocytic transport vesicle formation from the TGN (Jones, S.M., J.R. Crosby, J. Salamero, and K.E. Howell. 1993. J. Cell Biol. 122:775-788). Here the p62(cplx) is identified as a regulatory subunit of a novel phosphatidylinositol 3-kinase (PI3-kinase). This p62(cplx)-associated PI3-kinase activity is stimulated by activation of the p62(cplx)-associated GTPase, and is specific for phosphatidylinositol (PI) as substrate, and is sensitive to wortmannin at micromolar concentrations. The direct role of this p62(cplx)-associated PI3-kinase activity in TGN-derived vesicle formation is indicated by the finding that both lipid kinase activity and the formation of pIgA-R-containing exocytic vesicles from the TGN are inhibited by wortmannin with similar dose-response curves and 50% inhibitory concentrations (3.5 microM). These findings indicate that phosphatidylinositol-3-phosphate (PI[3]P) is required for the formation of TGN-derived exocytic transport vesicles, and that the p62(cplx)-associated PI3-kinase and an activated GTPase are the essential molecules that drive production of this PI(3)P.
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Registered trials
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