ArticleAntimicrobial agents and chemotherapy1997
Studies of the mechanism of human salivary histatin-5 candidacidal activity with histatin-5 variants and azole-sensitive and -resistant Candida species.
Article in Antimicrobial agents and chemotherapy, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 73 citations in OpenAlex.
- Optimizing Histatin 5: Effects of K13 and K17 Substitutions on Proteolytic Stability and Antifungal Activity.Computational and structural biotechnology journal · 2026Article
- Histatin 8 Interactions with Copper, Zinc, and Nickel Ions, and Its Antimicrobial Profile in Relation to Histatin 5.Molecules (Basel, Switzerland) · 2025Article
- Exploring the Healing Powers of Histatins: From Oral Health to Therapeutics.International journal of molecular sciences · 2025Review
- Effects of histatin 5 modifications on antifungal activity and kinetics of proteolysis.Protein science : a publication of the Protein Society · 2020Article
- Digital Design of a Universal Rat Intraoral Device for Therapeutic Evaluation of a Topical Formulation againstInfection and immunity · 2019Article
- Specific metallo-protein interactions and antimicrobial activity in Histatin-5, an intrinsically disordered salivary peptide.Scientific reports · 2019Article
- Antimicrobial Activity, Antioxidant Potential, Cytotoxicity and Phytochemical Profiling of Four Plants Locally Used against Skin Diseases.Plants (Basel, Switzerland) · 2019Article
- Histatin peptides: Pharmacological functions and their applications in dentistry.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2017Review
- The oral microbiome and the immunobiology of periodontal disease and caries.Immunology letters · 2014Review
- Candida albicans flu1-mediated efflux of salivary histatin 5 reduces its cytosolic concentration and fungicidal activity.Antimicrobial agents and chemotherapy · 2013Article
- Impaired Histatin-5 Levels and Salivary Antimicrobial Activity againstJournal of AIDS & clinical research · 2013Article
- Anti-candidal activity of genetically engineered histatin variants with multiple functional domains.PloS one · 2012Article
- Protection of the oral mucosa by salivary histatin-5 against Candida albicans in an ex vivo murine model of oral infection.FEMS yeast research · 2010Article
- Candidacidal activity of synthetic peptides based on the antimicrobial domain of the neutrophil-derived protein, CAP37.Medical mycology · 2010Article
- Sensitivity of Candida albicans biofilm cells grown on denture acrylic to antifungal proteins and chlorhexidine.Archives of oral biology · 2009Article
- The P-113 fragment of histatin 5 requires a specific peptide sequence for intracellular translocation in Candida albicans, which is independent of cell wall binding.Antimicrobial agents and chemotherapy · 2008Article
- Human salivary histatin 5 fungicidal action does not induce programmed cell death pathways in Candida albicans.Antimicrobial agents and chemotherapy · 2004Article
- Oral manifestations in HIV infection: fungal and bacterial infections, Kaposi's sarcoma.Medical microbiology and immunology · 2003Review
- MUC7 20-Mer: investigation of antimicrobial activity, secondary structure, and possible mechanism of antifungal action.Antimicrobial agents and chemotherapy · 2003Article
- Susceptibility of Candida dubliniensis to salivary histatin 3.Antimicrobial agents and chemotherapy · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Histatins are a group of small, cationic, antifungal peptides present in human saliva. A previous molecular modeling analysis suggested structural similarity between the Phe14-His15 and His18-His19 dipeptide sequences in histatin-5 (Hsn-5; a 24-amino-acid polypeptide) and the sequence of miconazole (one of the azole-based antifungal therapeutic agents), implying that the mechanisms of killing of Candida albicans by these two molecules may be similar. To further elaborate on this observation, we have produced two variants of Hsn-5 in which Phe14-His15 or His18-His19 dipeptide sequences were replaced by Ala-Ala (F14A/H15A and H18A/H19A) to eliminate the phenyl and imidazole rings of the side chains and assessed their candidacidal activities against C. albicans. In addition, we tested azole-resistant C. albicans and Candida glabrata strains for their susceptibilities to Hsn-5. Analysis of the purified recombinant proteins for their candidacidal activities indicated that both variants were significantly less effective (the molar concentrations required to kill half of the maximum number of cells [ED50s], approximately 67 and approximately 149 microM for F14A/H15A and H18A/H19A, respectively) than the unaltered Hsn-5 (ED50, approximately 8 microM) at killing C. albicans, suggesting that the two dipeptide sequences are important for the candidacidal activity of Hsn-5. Assessment of the candidacidal activity of Hsn-5 with the well-characterized azole-resistant strains of C. albicans and C. glabrata, however, suggested that the mode of action of histatins against Candida is distinct from that of azole-based antifungal agents because Hsn-5 kills both azole-sensitive and azole-resistant strains equally well.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.