ArticleProceedings of the National Academy of Sciences of the United States of America1997
Genetic definition of a protein-splicing domain: functional mini-inteins support structure predictions and a model for intein evolution.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 46 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 138 citations in OpenAlex.
- Harnessing Affinity Tags for Recombinant Protein Purification: Concepts, Methods, and Troubleshooting.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Conditional Split Inteins: Adaptable Tools for Programming Protein Functions.International journal of molecular sciences · 2025Review
- Inteins-mechanism of protein splicing, emerging regulatory roles, and applications in protein engineering.Frontiers in microbiology · 2023Review
- The Evolution of Intein-Based Affinity Methods as Reflected in 30 years of Patent History.Frontiers in molecular biosciences · 2022Review
- Structural Basis for the Propagation of Homing Endonuclease-Associated Inteins.Frontiers in molecular biosciences · 2022Article
- An alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein.Scientific reports · 2021Article
- Inteins in Science: Evolution to Application.Microorganisms · 2020Review
- Protein Splicing Activity of theBiochemistry · 2020Article
- Chemoenzymatic Semisynthesis of Proteins.Chemical reviews · 2020Review
- Biotechnological Applications of Protein Splicing.Current protein & peptide science · 2019Review
- Cisplatin protects mice from challenge ofEmerging microbes & infections · 2019Article
- Structural Basis for the Persistence of Homing Endonucleases in Transcription Factor IIB Inteins.Journal of molecular biology · 2017Article
- Dynamics differentiate between active and inactive inteins.European journal of medicinal chemistry · 2015Article
- Nature's recipe for splitting inteins.Protein engineering, design & selection : PEDS · 2014Review
- Protein splicing: how inteins escape from precursor proteins.The Journal of biological chemistry · 2014Review
- Enigmatic distribution, evolution, and function of inteins.The Journal of biological chemistry · 2014Review
- Engineering and optimization of an allosteric biosensor protein for peroxisome proliferator-activated receptor γ ligands.Biosensors & bioelectronics · 2011Article
- pK(a) coupling at the intein active site: implications for the coordination mechanism of protein splicing with a conserved aspartate.Journal of the American Chemical Society · 2011Article
- An intein with genetically selectable markers provides a new approach to internally label proteins with GFP.BMC biotechnology · 2011Article
- Cyclization of the intrinsically disordered α1S dihydropyridine receptor II-III loop enhances secondary structure and in vitro function.The Journal of biological chemistry · 2011Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Inteins are protein-splicing elements, most of which contain conserved sequence blocks that define a family of homing endonucleases. Like group I introns that encode such endonucleases, inteins are mobile genetic elements. Recent crystallography and computer modeling studies suggest that inteins consist of two structural domains that correspond to the endonuclease and the protein-splicing elements. To determine whether the bipartite structure of inteins is mirrored by the functional independence of the protein-splicing domain, the entire endonuclease component was deleted from the Mycobacterium tuberculosis recA intein. Guided by computer modeling studies, and taking advantage of genetic systems designed to monitor intein function, the 440-aa Mtu recA intein was reduced to a functional mini-intein of 137 aa. The accuracy of splicing of several mini-inteins was verified. This work not only substantiates structure predictions for intein function but also supports the hypothesis that, like group I introns, mobile inteins arose by an endonuclease gene invading a sequence encoding a small, functional splicing element.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.