ArticleProceedings of the National Academy of Sciences of the United States of America1997
DNA polymerase delta isolated from Schizosaccharomyces pombe contains five subunits.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 77 citations in OpenAlex.
- Review
- POLD3 as Controller of Replicative DNA Repair.International journal of molecular sciences · 2024Review
- Quaternary structural diversity in eukaryotic DNA polymerases: monomeric to multimeric form.Current genetics · 2020Review
- Pold3 is required for genomic stability and telomere integrity in embryonic stem cells and meiosis.Nucleic acids research · 2018Article
- Review
- POLD3 Is Haploinsufficient for DNA Replication in Mice.Molecular cell · 2016Article
- Loss of Pol32 in Drosophila melanogaster causes chromosome instability and suppresses variegation.PloS one · 2015Article
- Reconstitution of eukaryotic lagging strand DNA replication.Methods (San Diego, Calif.) · 2010Review
- Functional mapping of the fission yeast DNA polymerase delta B-subunit Cdc1 by site-directed and random pentapeptide insertion mutagenesis.BMC molecular biology · 2009Article
- Activation of the DNA damage checkpoint in mutants defective in DNA replication initiation.Molecular biology of the cell · 2008Article
- An in vivo analysis of the localisation and interactions of human p66 DNA polymerase delta subunit.BMC molecular biology · 2005Article
- Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase delta subunit Cdc27.BMC molecular biology · 2004Article
- The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit.Nucleic acids research · 2004Article
- Genetics of lagging strand DNA synthesis and maturation in fission yeast: suppression analysis links the Dna2-Cdc24 complex to DNA polymerase delta.Nucleic acids research · 2004Article
- The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Identification of the CAB2/hCOS16 gene required for the repair of DNA double-strand breaks on a core amplified region of the 17q12 locus in breast and gastric cancers.Japanese journal of cancer research : Gann · 2002Article
- Identification of delta helicase as the bovine homolog of HUPF1: demonstration of an interaction with the third subunit of DNA polymerase delta.Nucleic acids research · 2002Article
- Genetic analyses of Schizosaccharomyces pombe dna2(+) reveal that dna2 plays an essential role in Okazaki fragment metabolism.Genetics · 2000Article
- Functional interaction between the Werner Syndrome protein and DNA polymerase delta.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Essential interaction between the fission yeast DNA polymerase delta subunit Cdc27 and Pcn1 (PCNA) mediated through a C-terminal p21(Cip1)-like PCNA binding motif.The EMBO journal · 2000Article
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
DNA polymerase delta (pol delta) plays an essential role in DNA replication, repair, and recombination. We have purified pol delta from Schizosaccharomyces pombe more than 10(3)-fold and demonstrated that the polymerase activity of purified S. pombe pol delta is completely dependent on proliferating cell nuclear antigen and replication factor C. SDS/PAGE analysis of the purified fraction indicated that the pol delta complex consists of five subunits that migrate with apparent molecular masses of 125, 55, 54, 42, and 22 kDa. Western blot analysis indicated that the 125, 55, and 54 kDa proteins are the large catalytic subunit (Pol3), Cdc1, and Cdc27, respectively. The identity of the other two subunits, p42 and p22, was determined following proteolytic digestion and sequence analysis of the resulting peptides. The peptide sequences derived from the p22 subunit indicated that this subunit is identical to Cdm1, previously identified as a multicopy suppressor of the temperature-sensitive cdc1-P13 mutant, whereas peptide sequences derived from the p42 subunit were identical to a previously uncharacterized ORF located on S. pombe chromosome 1.
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