ArticleJournal of bacteriology1997
Regulation of the dnaK operon of Streptomyces coelicolor A3(2) is governed by HspR, an autoregulatory repressor protein.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 71 citations in OpenAlex.
- Dual-calibration RT-qPCR reveals distinct dnaK1/2/3 and groEL1/2 expression dynamics under heat and acid stress in the sinefungin producer Streptomyces incarnatus.Scientific reports · 2026Article
- ClpB enhances thermotolerance inMicrobiology spectrum · 2025Article
- Substrates bind to residues lining the ring of asymmetrically engaged bacterial proteasome activator Bpa.Nature communications · 2025Article
- A tunable and reversible thermo-inducible bio-switch for streptomycetes.Nucleic acids research · 2025Article
- Heat Shock Repressor HspR Directly Controls Avermectin Production, Morphological Development, and HApplied and environmental microbiology · 2021Article
- Survival in Hostile Conditions: Pupylation and the Proteasome in Actinobacterial Stress Response Pathways.Frontiers in molecular biosciences · 2021Review
- Cooperative Regulation ofMicroorganisms · 2020Article
- A comparison of key aspects of gene regulation in Streptomyces coelicolor and Escherichia coli using nucleotide-resolution transcription maps produced in parallel by global and differential RNA sequencing.Molecular microbiology · 2014Article
- Activating secondary metabolism with stress and chemicals.Journal of industrial microbiology & biotechnology · 2014Review
- Article
- The ROK family regulator Rok7B7 pleiotropically affects xylose utilization, carbon catabolite repression, and antibiotic production in streptomyces coelicolor.Journal of bacteriology · 2013Article
- HspR mutations are naturally selected in Bifidobacterium longum when successive heat shock treatments are applied.Journal of bacteriology · 2010Article
- An interactive regulatory network controls stress response in Bifidobacterium breve UCC2003.Journal of bacteriology · 2009Article
- Development and application of versatile high density microarrays for genome-wide analysis of Streptomyces coelicolor: characterization of the HspR regulon.Genome biology · 2009Article
- Molecular chaperones in pathogen virulence: emerging new targets for therapy.Cell host & microbe · 2008Review
- Regulation of the alpha-crystallin gene acr2 by the MprAB two-component system of Mycobacterium tuberculosis.Journal of bacteriology · 2007Article
- Thermosensors in eubacteria: role and evolution.Journal of biosciences · 2007Review
- Gene structure and transcriptional organization of the dnaK operon of Bifidobacterium breve UCC 2003 and application of the operon in bifidobacterial tracing.Applied and environmental microbiology · 2005Article
- Mycobacterium tuberculosis pathogenesis and molecular determinants of virulence.Clinical microbiology reviews · 2003Review
- Transcription of Clostridium cellulovorans cellulosomal cellulase and hemicellulase genes.Journal of bacteriology · 2003Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dnaK operon of Streptomyces coelicolor contains four genes (5'-dnaK-grpE-dnaJ-hspR). The fourth gene encodes a novel heat shock protein, HspR, which appears so far to be unique to the high-G+C actinomycete group of bacteria. HspR binds with high specificity to three inverted repeat sequences in the promoter region of the S. coelicolor dnaK operon, strongly suggesting a direct role for HspR in heat shock gene regulation. Here we present genetic and biochemical evidence that HspR is the repressor of the dnaK operon. Disruption of hspR leads to high-level constitutive transcription of the dnaK operon. Parallel transcriptional analyses of groESL1 and groEL2 expression demonstrated that heat shock regulation of the groE genes was essentially unaffected in an hspR null mutant, although the basal (uninduced) level of groEL2 transcription was slightly elevated compared with the wild type. The results of HspR titration experiments, where the dnaK operon promoter region was cloned at ca. 50 copies per chromosome, were consistent with the prediction that HspR functions as a negative autoregulator. His-tagged HspR, overproduced and purified from Escherichia coli, was shown to repress transcription from the dnaK operon promoter in vitro, providing additional evidence for the proposal that HspR directly regulates transcription of the dnaK operon. These studies indicate that there are at least two transcriptional mechanisms for controlling heat shock genes in S. coelicolor--one controlling the dnaK operon and another controlling the groE genes.
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