ArticleNucleic acids research1997
Alternative mRNA splice variants of the rat ClC-2 chloride channel gene are expressed in lung: genomic sequence and organization of ClC-2.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 29 citations in OpenAlex.
- Research and progress on ClC‑2 (Review).Molecular medicine reports · 2017Review
- The ClC-3 chloride channel and osmoregulation in the European sea bass, Dicentrarchus labrax.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2013Article
- A synthetic prostone activates apical chloride channels in A6 epithelial cells.American journal of physiology. Gastrointestinal and liver physiology · 2008Article
- Identification and functional characterization of a voltage-gated chloride channel and its novel splice variant in taste bud cells.The Journal of biological chemistry · 2005Article
- CLC-2 single nucleotide polymorphisms (SNPs) as potential modifiers of cystic fibrosis disease severity.BMC medical genetics · 2004Article
- Effect of an N-terminus deletion on voltage-dependent gating of the ClC-2 chloride channel.The Journal of physiology · 2002Article
- Inward-rectifying anion channels are expressed in the epithelial cells of choroid plexus isolated from ClC-2 'knock-out' mice.The Journal of physiology · 2002Article
- Expression of the voltage-gated chloride channel ClC-2 in rod bipolar cells of the rat retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Low expression of the ClC-2 chloride channel during postnatal development: a mechanism for the paradoxical depolarizing action of GABA and glycine in the hippocampus.Proceedings. Biological sciences · 1999Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The ClC-2 epithelial cell chloride channel is a voltage-, tonicity- and pH-regulated member of the ClC super family. We have previously shown that rat lung ClC-2 (rClC-2) is down-regulated at birth, and molecular diversity is generated by alternative splicing [Murray et al. (1995) Am. J. Respir. Cell Mol. Biol. 12, 597-604; Murray et al. (1996) Am. J. Physiol. 271, L829-L837; Chu et al . (1996) Nucleic Acids Res. 24, 3453-3457]. To investigate other possible mRNA splice variations, we sequenced the entire rClC-2 gene and found that ClC-2Sa (formerly ClC-2S) results from the deletion of exon 20. The preceding intron 19 has an unusually high CT content and a rare AAG acceptor site. Because both features were also found in intron 13, we next tested the hypothesis that intron 13 would be involved in alternative splicing. As predicted, a second splice product, ClC-2Sb, was found by RT-PCR, but only in lung. When we compared the genomic maps of rClC-2 and human ClC-1 (hClC-1), striking similarities were found in each exon except for rClC-2 exon 20, which is absent in hClC-1. These observations suggest that ClC-1 and ClC-2 may have evolved by gene duplication, mutation and DNA rearrangement.
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