ArticleNucleic acids research1997
A conserved core element is functionally important for maize mitochondrial promoter activity in vitro.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Major contribution of transcription initiation to 5'-end formation of mitochondrial steady-state transcripts in maize.RNA biology · 2019Article
- In vitro promoter recognition by the catalytic subunit of plant phage-type RNA polymerases.Plant molecular biology · 2016Article
- Phage-type RNA polymerase RPOTmp performs gene-specific transcription in mitochondria of Arabidopsis thaliana.The Plant cell · 2009Article
- The complete nucleotide sequence and multipartite organization of the tobacco mitochondrial genome: comparative analysis of mitochondrial genomes in higher plants.Molecular genetics and genomics : MGG · 2005Article
- Multiple promoters are a common feature of mitochondrial genes in Arabidopsis.Nucleic acids research · 2005Article
- RT-PCR analysis of 5' to 3'-end-ligated mRNAs identifies the extremities of cox2 transcripts in pea mitochondria.Nucleic acids research · 2002Article
- Characterization of a DNA-binding protein implicated in transcription in wheat mitochondria.Molecular and cellular biology · 1999Article
- Genomic context influences the activity of maize mitochondrial cox2 promoters.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Functional analysis of two maize cDNAs encoding T7-like RNA polymerases.The Plant cell · 1999Article
- In vitro characterization of the tobacco rpoB promoter reveals a core sequence motif conserved between phage-type plastid and plant mitochondrial promoters.The EMBO journal · 1999Article
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2 authors.
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Abstract
We have previously used a homologous in vitro transcription system to define functional elements of the maize mitochondrial atpA promoter. These elements comprise a central domain extending from -7 to +5, relative to the transcription start site, and an upstream domain of 1-3 bp that is purine rich and centered around positions -11 to -12. Within the central domain lies an essential 5 bp core element. These elements are conserved in many mitochondrial promoters, but their functionality has only been tested for atpA. In this study we have introduced mutations into the corresponding elements of two cox3 promoters and show that while the core element is essential for cox3 promoter activity, upstream element mutations have little or no effect. To define the minimal sequence required for in vitro promoter activity a series of short cloned oligonucleotides corresponding to the atpA promoter was used. While some activity was seen with a 14 bp sequence, full activity required 26 bp, suggesting that elements other than the core and upstream region can influence promoter strength. Another series of clones showed that altered spacing between the upstream and core elements of atpA had a significant effect on promoter activity. These results further define important features of the plant mitochondrial transcriptional machinery.
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