ArticleThe EMBO journal1997
Regulation of ES cell differentiation by functional and conformational modulation of p53.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
74 citing papers in PubMed, 155 citations in OpenAlex.
- p53 prophylactic therapy for cancer prevention.Cell death and differentiation · 2025Article
- Crosstalk between CaInternational journal of molecular sciences · 2022Review
- Transient upregulation of IRF1 during exit from naive pluripotency confers viral protection.EMBO reports · 2022Article
- p53 inactivation unmasks histone methylation-independent WDR5 functions that drive self-renewal and differentiation of pluripotent stem cells.Stem cell reports · 2021Article
- Ubiquitous Chromatin Modifiers in Congenital Retinal Diseases: Implications for Disease Modeling and Regenerative Medicine.Trends in molecular medicine · 2021Review
- p53 Integrates Temporal WDR5 Inputs during Neuroectoderm and Mesoderm Differentiation of Mouse Embryonic Stem Cells.Cell reports · 2020Article
- Cancer Stemness: p53 at the Wheel.Frontiers in oncology · 2020Review
- E3 ubiquitin ligase MDM2 acts through p53 to control respiratory progenitor cell number and lung size.Development (Cambridge, England) · 2019Article
- p53 balances between tissue hierarchy and anarchy.Journal of molecular cell biology · 2019Review
- Amniotic Fluid Cells, Stem Cells, and p53: Can We Stereotype p53 Functions?International journal of molecular sciences · 2019Review
- Nucleotide excision repair capacity increases during differentiation of human embryonic carcinoma cells into neurons and muscle cells.The Journal of biological chemistry · 2019Article
- Understanding p53 functions through p53 antibodies.Journal of molecular cell biology · 2019Review
- p53 Is Active in Human Amniotic Fluid Stem Cells.Stem cells and development · 2018Article
- Article
- Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells.Scientific reports · 2017Article
- Emerging Non-Canonical Functions and Regulation by p53: p53 and Stemness.International journal of molecular sciences · 2016Review
- The Paradox of p53: What, How, and Why?Cold Spring Harbor perspectives in medicine · 2016Review
- Histone modifications and p53 binding poise the p21 promoter for activation in human embryonic stem cells.Scientific reports · 2016Article
- G1 checkpoint is compromised in mouse ESCs due to functional uncoupling of p53-p21Waf1 signaling.Cell cycle (Georgetown, Tex.) · 2016Article
- The concept of radiation-enhanced stem cell differentiation.Radiology and oncology · 2015Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic stem (ES) cell lines were used to examine the role of p53 during in vitro differentiation. Undifferentiated ES cells express high levels of p53 exclusively in the wild-type conformation, immunoprecipitable by monoclonal antibody PAb246, and p53 was found to be functionally active as determined by its ability to bind DNA specifically and to activate transcription of target genes. Differentiation in vitro resulted in a decrease in the levels of p53 and in a shift in its conformational status to the mutant form, detectable by monoclonal antibody PAb240, with a concomitant loss of functional activity. The presence of functional p53 in the undifferentiated ES cells renders them hypersensitive to UV irradiation, whereas the differentiated cells were resistant to UV treatment. ES cells lacking p53 exhibit enhanced proliferation in both the undifferentiated and differentiated state, and apoptosis accompanying differentiation was found to be reduced. Furthermore, wild-type ES cells undergoing apoptosis expressed functional p53. Expression of the temperature-sensitive p53val135 mutant in wild-type ES cells resulted in a reduction of apoptosis accompanying differentiation when it adopted a mutant conformation at 39 degrees C. These data demonstrate that functional inactivation of p53 allows differentiating cells to escape from apoptosis, and suggest that the conformational switch could regulate the inactivation process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.