ArticleInfection and immunity1997
Haemophilus somnus immunoglobulin binding proteins and surface fibrils.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 52 citations in OpenAlex.
- Article
- Evolving views on bovine respiratory disease: An appraisal of selected key pathogens - Part 1.Veterinary journal (London, England : 1997) · 2016Review
- Histophilus somni Stimulates Expression of Antiviral Proteins and Inhibits BRSV Replication in Bovine Respiratory Epithelial Cells.PloS one · 2016Article
- The role of lipooligosaccharide phosphorylcholine in colonization and pathogenesis of Histophilus somni in cattle.Veterinary research · 2012Article
- Horizontal gene transfer in Histophilus somni and its role in the evolution of pathogenic strain 2336, as determined by comparative genomic analyses.BMC genomics · 2011Article
- Histophilus somni IbpA DR2/Fic in virulence and immunoprotection at the natural host alveolar epithelial barrier.Infection and immunity · 2010Article
- AMPylation: Something Old is New Again.Frontiers in microbiology · 2010Article
- Molecular characterization of phosphorylcholine expression on the lipooligosaccharide of Histophilus somni.Microbial pathogenesis · 2009Article
- Protection of mice against H. somni septicemia by vaccination with recombinant immunoglobulin binding protein subunits.Vaccine · 2008Article
- Platelet activation by Histophilus somni and its lipooligosaccharide induces endothelial cell proinflammatory responses and platelet internalization.Shock (Augusta, Ga.) · 2008Article
- Characterization and comparison of biofilm development by pathogenic and commensal isolates of Histophilus somni.Journal of bacteriology · 2007Article
- Roles of cellular activation and sulfated glycans in Haemophilus somnus adherence to bovine brain microvascular endothelial cells.Infection and immunity · 2006Article
- Expression of the LspA1 and LspA2 proteins by Haemophilus ducreyi is required for virulence in human volunteers.Infection and immunity · 2004Article
- Mutations in the lspA1 and lspA2 genes of Haemophilus ducreyi affect the virulence of this pathogen in an animal model system.Infection and immunity · 2003Article
- Haemophilus somnus induces apoptosis in bovine endothelial cells in vitro.Infection and immunity · 2001Article
- Characterization of putative Haemophilus somnus isolates from tonsils of American bison (Bison bison).Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The high-molecular-weight (HMW) immunoglobulin binding proteins (IgBPs) of Haemophilus somnus and a 76-kDa surface protein (p76) are found in serum-resistant virulent strains but not in several serum-sensitive strains from asymptomatic carriers. For the first time, p76 was shown to be an IgBP also. This was done by competitive inhibition studies with affinity-purified antidinitrophenol (anti-DNP) and DNP to ensure that binding was not antigen specific. The HMW IgBPs, but not the p76 IgBP, were partially purified from concentrated culture supernatant in detergent by fluid-phase liquid chromatography with a gel filtration column. Membrane extraction studies showed that p76 predominated in the Sarkosyl-soluble fraction of the bacterial cell pellet. Since integral outer membrane (OM) proteins are Sarkosyl insoluble, this is consistent with our previous finding that implicated p76 as a peripheral OM protein. The HMW IgBPs were found predominantly in the Sarkosyl-soluble fraction of the culture supernatant. This suggests that they were not integral membrane proteins and that their presence in the supernatant was not due to OM blebbing. We then showed that two IgBP-positive serum-resistant virulent strains have a surface fibrillar network but that two IgBP-negative serum-sensitive H. somnus strains from asymptomatic preputial carriers do not. Fibrils on the surfaces of IgBP+ strains bound gold-labelled bovine immunoglobulin G2 (IgG2) anti-DNP, indicating that these fibrils have IgG2 binding activity. Therefore, this study shows that H. somnus has two IgBPs, including a peripheral membrane protein and a fibrillar surface network.
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