ArticleInfection and immunity1997
Characterization of a methyl-accepting chemotaxis protein gene, dmcA, from the oral spirochete Treponema denticola.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 40 citations in OpenAlex.
- Porphyromonas gingivalis Outer Membrane Vesicles Mediate Coaggregation and Piggybacking of Treponema denticola and Lachnoanaerobaculum saburreum.International journal of dentistry · 2013Article
- Treponema denticola interactions with host proteins.Journal of oral microbiology · 2012Article
- Virulence factors of the oral spirochete Treponema denticola.Journal of dental research · 2011Review
- CheX is a phosphorylated CheY phosphatase essential for Borrelia burgdorferi chemotaxis.Journal of bacteriology · 2005Article
- Construction and characterization of a cheA mutant of Treponema denticola.Journal of bacteriology · 2002Article
- Asymmetrical flagellar rotation in Borrelia burgdorferi nonchemotactic mutants.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Complementation of a Treponema denticola flgE mutant with a novel coumermycin A1-resistant T. denticola shuttle vector system.Infection and immunity · 2002Article
- Motility and chemotaxis in tissue penetration of oral epithelial cell layers by Treponema denticola.Infection and immunity · 2001Article
- Cloning and expression of two novel hemin binding protein genes from Treponema denticola.Infection and immunity · 2001Article
- Characterization of a novel methyl-accepting chemotaxis gene, dmcB, from the oral spirochete Treponema denticola.Infection and immunity · 1999Article
- Molecular characterization of Treponema pallidum mcp2, a putative chemotaxis protein gene.Infection and immunity · 1998Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A gene, dmcA, expressing a methyl-accepting chemotaxis protein (MCP) from the oral spirochete Treponema denticola has been characterized. The gene was initially identified as an open reading frame immediately upstream from the previously characterized prtB protease gene of strain ATCC 35405. Nucleotide sequencing of the dmcA gene revealed a potential 57-kDa protein product with extensive homology with the signaling regions of MCPs from a variety of bacteria. The protein expressed in Escherichia coli cross-reacted with anti-Trg (E. coli MCP) serum, confirming its homology with MCPs. Northern blot and primer extension analyses identified the transcription start site of the monocistronic dmcA mRNA. By utilizing a T. denticola gene inactivation system recently developed in this laboratory, a mutant defective in the dmcA gene, HL0501, was constructed. The mutant was demonstrated to be defective in chemotaxis toward nutrients. In addition, the methylation profiles of cellular proteins indicated altered MCPs in the mutant relative to those of the parental strain. These results indicate that we have identified an MCP gene in the oral spirochete which plays a significant role in the chemotactic response of the organism.
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Registered trials
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