ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1997
Active polysomes are present in the large presynaptic endings of the synaptosomal fraction from squid brain.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 67 citations in OpenAlex.
- Functional Genomics of Axons and Synapses to Understand Neurodegenerative Diseases.Frontiers in cellular neuroscience · 2021Review
- Translational Control in the Brain in Health and Disease.Cold Spring Harbor perspectives in biology · 2019Review
- DNA in Squid Synaptosomes.Molecular neurobiology · 2019Article
- Interrogating Synaptic Architecture: Approaches for Labeling Organelles and Cytoskeleton Components.Frontiers in synaptic neuroscience · 2019Review
- Squid Giant Axons Synthesize NF Proteins.Molecular neurobiology · 2018Article
- Microtubule-dependent ribosome localization ineLife · 2017Article
- Intra-axonal protein synthesis in development and beyond.International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience · 2016Review
- The local expression and trafficking of tyrosine hydroxylase mRNA in the axons of sympathetic neurons.RNA (New York, N.Y.) · 2016Article
- Ultrastructure of synapses in the mammalian brain.Cold Spring Harbor perspectives in biology · 2012Review
- Axonal mRNA localization and local protein synthesis in nervous system assembly, maintenance and repair.Nature reviews. Neuroscience · 2012Review
- Regulation of protein levels in subcellular domains through mRNA transport and localized translation.Molecular & cellular proteomics : MCP · 2010Article
- RNA translation in axons.Annual review of cell and developmental biology · 2004Review
- Transport of Neuronal BC1 RNA in Mauthner Axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 5 institutions in 5 countries.
Funding
Abstract
Previous data have suggested that the large nerve terminals present in the synaptosomal fraction from squid optic lobe are capable of protein synthesis (Crispino et al., 1993a,b). We have further examined this issue by comparing the translation products of synaptosomal and microsomal polysomes. Both preparations programmed an active process of translation, which was completely abolished by their previous treatment with EDTA. After immunoabsorption of the newly synthesized neurofilament (NF) proteins, the labeling ratio of the 60 and 70 kDa NF proteins was found to differ, in agreement with comparable differences obtained with intact synaptosomes. These observations indicate that the set of mRNAs translated by synaptosomes differs from that translated by nerve cell bodies. Hence, because NF proteins are neuron-specific, they support the view that the active synaptosomal polysomes are mostly localized in the large nerve terminals that represent the most abundant neuronal component of the fraction. This hypothesis was confirmed (1) by electron spectroscopic data demonstrating the presence of ribosomes and polysomes within the large nerve endings of the synaptosomal fraction, as well as in the carrot-like nerve endings of the retinal photoreceptors that constitute the only large terminals in the optic lobe, and (2) by light and high resolution autoradiography of synaptosomal samples incubated with [3H]leucine, showing that most labeled proteins are associated with the large nerve endings. This response was abolished by cycloheximide. Taken together, the data provide the first unequivocal demonstration that presynaptic nerve terminals are capable of protein synthesis.
Indexed as
Identifiers
9315891PMC6793907W2118629625What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.