ArticleMolecular and cellular biology1997
Developmental regulation of DNA replication: replication fork barriers and programmed gene amplification in Tetrahymena thermophila.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Developmentally Programmed Switches in DNA Replication: Gene Amplification and Genome-Wide Endoreplication inMicroorganisms · 2023Article
- Checkpoint Activation of an Unconventional DNA Replication Program in Tetrahymena.PLoS genetics · 2015Article
- Developmental regulation of the Tetrahymena thermophila origin recognition complex.PLoS genetics · 2015Article
- Impaired replication elongation in Tetrahymena mutants deficient in histone H3 Lys 27 monomethylation.Genes & development · 2013Article
- Transcription-replication encounters, consequences and genomic instability.Nature structural & molecular biology · 2013Review
- Repeat expansion in the budding yeast ribosomal DNA can occur independently of the canonical homologous recombination machinery.Nucleic acids research · 2011Article
- Extreme polyploidy in a large bacterium.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition.The EMBO journal · 2007Article
- Article
- Replication fork stalling at natural impediments.Microbiology and molecular biology reviews : MMBR · 2007Review
- Deletion of the Tetrahymena thermophila rDNA replication fork barrier region disrupts macronuclear rDNA excision and creates a fragile site in the micronuclear genome.Nucleic acids research · 2006Article
- The mating type switch-activating protein Sap1 Is required for replication fork arrest at the rRNA genes of fission yeast.Molecular and cellular biology · 2005Article
- DNA replication origin plasticity and perturbed fork progression in human inverted repeats.Molecular and cellular biology · 2005Article
- TIF1 Represses rDNA replication initiation, but promotes normal S phase progression and chromosome transmission in Tetrahymena.Molecular biology of the cell · 2005Article
- Mechanisms of transcription-replication collisions in bacteria.Molecular and cellular biology · 2005Article
- Transcription termination factor reb1p causes two replication fork barriers at its cognate sites in fission yeast ribosomal DNA in vivo.Molecular and cellular biology · 2004Article
- Complex mechanism of site-specific DNA replication termination in fission yeast.The EMBO journal · 2003Article
- Characterization of a novel origin recognition complex-like complex: implications for DNA recognition, cell cycle control, and locus-specific gene amplification.Molecular and cellular biology · 2003Article
- Origin usage during euplotes ribosomal DNA amplification.Eukaryotic cell · 2003Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The palindromic Tetrahymena ribosomal DNA (rDNA) minichromosome is amplified 10,000-fold during development. Subsequent vegetative replication is cell cycle regulated. rDNA replication differs fundamentally in cycling vegetative and nondividing amplifying cells. Using two-dimensional gel electrophoresis, we show for the first time that replication origins that direct gene amplification also function in normal dividing cells. Two classes of amplification intermediates were identified. The first class is indistinguishable from vegetative rDNA, initiating in just one of the two 5' nontranscribed spacer (NTS) copies in the rDNA palindrome at either of two closely spaced origins. Thus, these origins are active throughout the life cycle and their regulation changes at different developmental stages. The second, novel class of amplification intermediates is generated by multiple initiation events. Intermediates with mass greater than fully replicated DNA were observed, suggesting that onionskin replication occurs at this stage. Unlike amplified rDNA in Xenopus laevis, the novel Tetrahymena species are not produced by random initiation; replication also initiates in the 5' NTS. Surprisingly, a replication fork barrier which is activated only in these amplifying molecules blocks the progression of forks near the center of the palindrome. Whereas barriers have been previously described, this is the first instance in which programmed regulation of replication fork progression has been demonstrated in a eukaryote.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.