ArticleMolecular and cellular biology1997
Stability of patch methylation and its impact in regions of transcriptional initiation and elongation.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
33 citing papers in PubMed, 77 citations in OpenAlex.
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- Effect of Methylation on Local Mechanics and Hydration Structure of DNA.Biophysical journal · 2018Article
- Influence of DNA methylation on the expression of OPG/RANKL in primary osteoporosis.International journal of medical sciences · 2018Article
- Low salinity affects cellularity, DNA methylation, and mRNA expression of igf1 in the liver of half smooth tongue sole (Cynoglossus semilaevis).Fish physiology and biochemistry · 2017Article
- Increasing the CpG dinucleotide abundance in the HIV-1 genomic RNA inhibits viral replication.Retrovirology · 2017Article
- Effect of CpG dinucleotides within IgH switch region repeats on immunoglobulin class switch recombination.Molecular immunology · 2015Article
- FXR silencing in human colon cancer by DNA methylation and KRAS signaling.American journal of physiology. Gastrointestinal and liver physiology · 2014Article
- Aberrantly hypermethylated Homeobox A2 derepresses metalloproteinase-9 through TBP and promotes invasion in Nasopharyngeal carcinoma.Oncotarget · 2013Article
- IL-2/IL-15 activate the human clonally restricted KIR3DL1 reverse promoter.Genes and immunity · 2013Article
- The effect of methylated oligonucleotide targeting Ki-67 gene in human 786-0 renal carcinoma cells.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2011Article
- Adeno-associated viral vector-mediated transgene expression is independent of DNA methylation in primate liver and skeletal muscle.PloS one · 2011Article
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- Interplay between DNA methylation and transcription factor availability: implications for developmental activation of the mouse Myogenin gene.Molecular and cellular biology · 2010Article
- Preferential epigenetic suppression of the autonomous MusD over the nonautonomous ETn mouse retrotransposons.Molecular and cellular biology · 2009Article
- The Igf2/H19 imprinting control region exhibits sequence-specific and cell-type-dependent DNA methylation-mediated repression.Nucleic acids research · 2009Article
- Selection against PUMA gene expression in Myc-driven B-cell lymphomagenesis.Molecular and cellular biology · 2008Article
- Postintegrative gene silencing within the Sleeping Beauty transposition system.Molecular and cellular biology · 2007Article
- Frequent but borderline methylation of p16 (INK4a) and TIMP3 in medulloblastoma and sPNET revealed by quantitative analyses.Journal of neuro-oncology · 2007Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
CpG DNA methylation has previously been correlated with the suppression of transcription. The mechanism of this suppression is not understood, and many aspects of the temporal and positional relationships between the region of methylation and transcription have not yet been defined. Here, 12-kb stable replicating episomes that can be maintained in human somatic cells for weeks to months were used. Such a system allows more direct manipulation and is free from the positional effects attendant with the analysis of endogenous loci or integrated transgenes. By using these circular minichromosomes, patches of CpG methylation were created to include or exclude the regions of transcriptional initiation and elongation. I found that a 0.5-kb patch of methylation that covered the promoter suppressed expression only 2-fold and that a 1.9-kb patch of methylation that covered the coding portion of the gene (but not the promoter) suppressed expression about 10-fold. In contrast, methylation of the entire minichromosome except for the promoter or the coding portion suppressed transcription about 50- to 200-fold. I infer the following. Methylation of the 0.5-kb promoter fragment does not significantly affect transcription at the level of transcription factor binding or local chromatin structure. The dominant effect on transcription occurs when the length of methylated DNA is long, with little disproportionate effect of methylation of specific regions, such as that of initiation or elongation. I also found that the boundaries between these methylated and unmethylated regions remained stable for the many weeks that I monitored them.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.