ArticleMolecular and cellular biology1997
Characterization of Cdc47p-minichromosome maintenance complexes in Saccharomyces cerevisiae: identification of Cdc45p as a subunit.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Open architecture of archaea MCM and dsDNA complexes resolved using monodispersed streptavidin affinity CryoEM.Nature communications · 2024Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Review
- Functional conservation of the pre-sensor one beta-finger hairpin (PS1-hp) structures in mini-chromosome maintenance proteins of Saccharomyces cerevisiae and archaea.G3 (Bethesda, Md.) · 2014Article
- MCM Paradox: Abundance of Eukaryotic Replicative Helicases and Genomic Integrity.Molecular biology international · 2014Review
- The cyclin A centrosomal localization sequence recruits MCM5 and Orc1 to regulate centrosome reduplication.Journal of cell science · 2010Article
- Solution NMR structure of the C-terminal DNA binding domain of Mcm10 reveals a conserved MCM motif.The Journal of biological chemistry · 2010Article
- The Mcm complex: unwinding the mechanism of a replicative helicase.Microbiology and molecular biology reviews : MMBR · 2009Review
- Article
- Cdc7-Dbf4 phosphorylates MCM proteins via a docking site-mediated mechanism to promote S phase progression.Molecular cell · 2006Article
- DNA replication and progression through S phase.Oncogene · 2005Review
- Eukaryotic MCM proteins: beyond replication initiation.Microbiology and molecular biology reviews : MMBR · 2004Review
- Human Mcm proteins at a replication origin during the G1 to S phase transition.Nucleic acids research · 2002Article
- SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast.Molecular biology of the cell · 2002Article
- Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints.Genetics · 2001Article
- Requirement of CDC45 for postimplantation mouse development.Molecular and cellular biology · 2001Article
- Localisation of the DmCdc45 DNA replication factor in the mitotic cycle and during chorion gene amplification.Nucleic acids research · 2000Article
- DNA synthesis at individual replication forks requires the essential initiation factor Cdc45p.The EMBO journal · 2000Article
- Assembly of a complex containing Cdc45p, replication protein A, and Mcm2p at replication origins controlled by S-phase cyclin-dependent kinases and Cdc7p-Dbf4p kinase.Molecular and cellular biology · 2000Article
- Mcm10 and the MCM2-7 complex interact to initiate DNA synthesis and to release replication factors from origins.Genes & development · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cdc47p is a member of the minichromosome maintenance (MCM) family of polypeptides, which have a role in the early stages of chromosomal DNA replication. Here, we show that Cdc47p assembles into stable complexes with two other members of the MCM family, Cdc46p and Mcm3p. The assembly of Cdc47p into complexes with Cdc46p does not appear to be cell cycle regulated, making it unlikely that these interactions per se are a rate-limiting step in the control of S phase. Cdc45p is also shown to interact with Cdc47p in vivo and to be a component of high-molecular-weight MCM complexes in cell lysates. Like MCM polypeptides, Cdc45p is essential for the initiation of chromosomal DNA replication in Saccharomyces cerevisiae; however, Cdc45p remains in the nucleus throughout the cell cycle, whereas MCMs are nuclear only during G1. We characterize two mutations in CDC47 and CDC46 which arrest cells with unduplicated DNA as a result of single base substitutions. The corresponding amino acid substitutions in Cdc46p and Cdc47p severely reduce the ability of these polypeptides to assemble in a complex with each other in vivo and in vitro. This argues that assembly of Cdc47p into complexes with other MCM polypeptides is important for its role in the initiation of chromosomal DNA replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.