ArticleMolecular and cellular biology1997
A carboxy-terminal basic region controls RNA polymerase III transcription factor activity of human La protein.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 70 citations in OpenAlex.
- Article
- AbioRxiv : the preprint server for biology · 2024Article
- Role of the RNA-binding protein La in cancer pathobiology.RNA biology · 2021Review
- An interdomain bridge influences RNA binding of the human La protein.The Journal of biological chemistry · 2019Article
- Human La binds mRNAs through contacts to the poly(A) tail.Nucleic acids research · 2018Article
- Article
- Novel RNA chaperone domain of RNA-binding protein La is regulated by AKT phosphorylation.Nucleic acids research · 2015Article
- Human La protein interaction with GCAC near the initiator AUG enhances hepatitis C Virus RNA replication by promoting linkage between 5' and 3' untranslated regions.Journal of virology · 2013Article
- La-motif-dependent mRNA association with Slf1 promotes copper detoxification in yeast.RNA (New York, N.Y.) · 2012Article
- RNA chaperone activity of human La protein is mediated by variant RNA recognition motif.The Journal of biological chemistry · 2012Article
- Precursor-product discrimination by La protein during tRNA metabolism.Nature structural & molecular biology · 2009Article
- Structural determinant of human La protein critical for internal initiation of translation of hepatitis C virus RNA.Journal of virology · 2008Article
- Cellular La protein shields nonsegmented negative-strand RNA viral leader RNA from RIG-I and enhances virus growth by diverse mechanisms.Journal of virology · 2008Article
- Structural analysis reveals conformational plasticity in the recognition of RNA 3' ends by the human La protein.Structure (London, England : 1993) · 2008Article
- Mouse and human La proteins differ in kinase substrate activity and activation mechanism for tRNA processing.Gene expression · 2007Article
- Human La is found at RNA polymerase III-transcribed genes in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Mammalian peptidylglycine alpha-amidating monooxygenase mRNA expression can be modulated by the La autoantigen.Molecular and cellular biology · 2005Article
- A peptide derived from RNA recognition motif 2 of human la protein binds to hepatitis C virus internal ribosome entry site, prevents ribosomal assembly, and inhibits internal initiation of translation.Journal of virology · 2005Article
- Nonphosphorylated human La antigen interacts with nucleolin at nucleolar sites involved in rRNA biogenesis.Molecular and cellular biology · 2004Article
- A peptide from autoantigen La blocks poliovirus and hepatitis C virus cap-independent translation and reveals a single tyrosine critical for La RNA binding and translation stimulation.Journal of virology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human La protein has been shown to serve as a transcription factor for RNA polymerase III (pol III) by facilitating transcription termination and recycling of transcription complexes. In addition, La binds to the 3' oligo(U) ends common to all nascent pol III transcripts, and in the case of B1-Alu RNA, protects it from 3'-end processing (R. J. Maraia, D. J. Kenan, and J. D. Keene, Mol. Cell. Biol. 14:2147-2158, 1994). Others have previously dissected the La protein into an N-terminal domain that binds RNA and a C-terminal domain that does not. Here, deletion and substitution mutants of La were examined for general RNA binding, RNA 3'-end protection, and transcription factor activity. Although some La mutants altered in a C-terminal basic region bind RNA in mobility shift assays, they are defective in RNA 3'-end protection and do not support transcription, while one C-terminal substitution mutant is defective only in transcription. Moreover, a C-terminal fragment lacking RNA binding activity appears able to support low levels of transcription by pol III. While efficient multiround transcription is supported only by mutants that bind RNA and contain a C-terminal basic region. These analyses indicate that RNA binding contributes to but is not sufficient for La transcription factor activity and that the C-terminal domain plays a role in transcription that is distinguishable from simple RNA binding. The transcription factor activity of La can be reversibly inhibited by RNA, suggesting the potential for feedback inhibition of pol III transcription.
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