ArticleThe EMBO journal1997
Bacteriophage phi29 DNA replication arrest caused by codirectional collisions with the transcription machinery.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Combinatorial Optimization of Protein Systems in Synthetic Cells.ACS synthetic biology · 2026Article
- A synthetic cell with integrated DNA self-replication and lipid biosynthesis.Nature communications · 2026Article
- Participants in Transcription-Replication Conflict and Their Role in Formation and Resolution of R-Loops.International journal of molecular sciences · 2025Review
- Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles.ACS synthetic biology · 2023Article
- Self-replication of DNA by its encoded proteins in liposome-based synthetic cells.Nature communications · 2018Article
- Review
- RecG Directs DNA Synthesis during Double-Strand Break Repair.PLoS genetics · 2016Article
- A transcription and translation-coupled DNA replication system using rolling-circle replication.Scientific reports · 2015Article
- Molecular traffic jams on DNA.Annual review of biophysics · 2013Review
- Back to the origin: reconsidering replication, transcription, epigenetics, and cell cycle control.Genes & cancer · 2012Article
- Replication-transcription conflicts in bacteria.Nature reviews. Microbiology · 2012Review
- Article
- Article
- Highly transcribed RNA polymerase II genes are impediments to replication fork progression in Saccharomyces cerevisiae.Molecular cell · 2009Article
- RecQ and RecG helicases have distinct roles in maintaining the stability of polypurine.polypyrimidine sequences.Mutation research · 2008Article
- Single-stranded DNA-binding protein in vitro eliminates the orientation-dependent impediment to polymerase passage on CAG/CTG repeats.The Journal of biological chemistry · 2008Article
- Genome-wide coorientation of replication and transcription reduces adverse effects on replication in Bacillus subtilis.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Replication fork stalling at natural impediments.Microbiology and molecular biology reviews : MMBR · 2007Review
- Transcription regulatory elements are punctuation marks for DNA replication.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Impairment of replication fork progression mediates RNA polII transcription-associated recombination.The EMBO journal · 2005Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The consequences on replication of collisions between phi29 DNA polymerase, a monomeric replicase endowed with strand displacement capacity, and the transcription machinery have been studied in vitro. Codirectional collisions with stalled transcription ternary complexes at four different promoters in the phi29 genome were found to block replication fork progression. Upon collision, the DNA polymerase remained on the template and was able to resume elongation once the RNA polymerase was allowed to move. Collisions with RNA polymerase molecules moving in the same direction also interfered with replication, causing a decrease in the replication rate. These results lead to the proposal that in bacteriophage phi29 a transcription complex physically blocks the progression of a replication fork. We suggest that temporal regulation of transcription and the low probability that the replication and transcription processes colocalize in vivo contribute to achieving minimal interference between the two events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.