ArticleThe EMBO journal1997
p45 NF-E2 regulates expression of thromboxane synthase in megakaryocytes.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 76 citations in OpenAlex.
- Redox Regulation of Megakaryocyte Differentiation and Platelet Biogenesis.Antioxidants (Basel, Switzerland) · 2026Review
- Nfe2 is dispensable for early but required for adult thrombocyte formation and function in zebrafish.Blood advances · 2018Article
- The KEAP1-NRF2 System: a Thiol-Based Sensor-Effector Apparatus for Maintaining Redox Homeostasis.Physiological reviews · 2018Review
- A novel mode of stimulating platelet formation activity in megakaryocytes with peanut skin extract.Journal of natural medicines · 2018Article
- NF-E2, FLI1 and RUNX1 collaborate at areas of dynamic chromatin to activate transcription in mature mouse megakaryocytes.Scientific reports · 2016Article
- Regulation and function of the NFE2 transcription factor in hematopoietic and non-hematopoietic cells.Cellular and molecular life sciences : CMLS · 2015Review
- NF-E2 p45 is important for establishing normal function of platelets.Molecular and cellular biology · 2013Article
- Molecular determinants for small Maf protein control of platelet production.Molecular and cellular biology · 2011Article
- Genetic analysis of hierarchical regulation for Gata1 and NF-E2 p45 gene expression in megakaryopoiesis.Molecular and cellular biology · 2010Article
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- A critical role for the transcription factor Scl in platelet production during stress thrombopoiesis.Blood · 2006Article
- MafG sumoylation is required for active transcriptional repression.Molecular and cellular biology · 2006Article
- Physical association of the patient-specific GATA1 mutants with RUNX1 in acute megakaryoblastic leukemia accompanying Down syndrome.Leukemia · 2006Article
- The biogenesis of platelets from megakaryocyte proplatelets.The Journal of clinical investigation · 2005Review
- Targeted disruption of Nrf2 causes regenerative immune-mediated hemolytic anemia.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Proplatelet formation of megakaryocytes is triggered by autocrine-synthesized estradiol.Genes & development · 2003Article
- Picosecond-hetero-FRET microscopy to probe protein-protein interactions in live cells.Biophysical journal · 2002Article
- Nuclear factor-erythroid 2 (NF-E2) expression in normal and malignant megakaryocytopoiesis.Leukemia · 2002Article
- Molecular control of megakaryopoiesis and thrombopoiesis.International journal of hematology · 2002Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription factor p45 NF-E2 is highly expressed in the erythroid and megakaryocytic lineages. Although p45 recognizes regulatory regions of several erythroid genes, mice deficient for this protein display only mild dyserythropoiesis but have abnormal megakaryocytes and lack circulating platelets. A number of megakaryocytic marker genes have been extensively studied, but none of them is regulated by NF-E2. To find target genes for p45 NF-E2 in megakaryopoiesis, we used an in vivo immunoselection assay: genomic fragments bound to p45 NF-E2 in the chromatin of a megakaryocytic cell line were immunoprecipitated with an anti-p45 antiserum and cloned. One of these fragments belongs to the second intron of the thromboxane synthase gene (TXS). We demonstrate that the TXS gene, which is mainly expressed in megakaryocytes, is indeed directly regulated by p45 NF-E2. First, its promoter contains a functional NF-E2 binding site; second, the intronic NF-E2 binding site is located within a chromatin-dependent enhancer element; third, p45-null murine megakaryocytes do not express detectable TXS mRNA, although TXS expression can be detected in other cells. These data, and the structure of the TXS promoter and enhancer, suggest that TXS belongs to a distinct subgroup of genes involved in platelet formation and function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.