ArticleThe EMBO journal1997
A member of the Ste20/PAK family of protein kinases is involved in both arrest of Xenopus oocytes at G2/prophase of the first meiotic cell cycle and in prevention of apoptosis.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- PAK1 Regulates MEC-17 Acetyltransferase Activity and Microtubule Acetylation during Proplatelet Extension.International journal of molecular sciences · 2020Article
- Article
- Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation.Nature communications · 2015Article
- Role of caspase-3 cleaved IP3 R1 on Ca(2+) homeostasis and developmental competence of mouse oocytes and eggs.Journal of cellular physiology · 2014Article
- Combined p21-activated kinase and farnesyltransferase inhibitor treatment exhibits enhanced anti-proliferative activity on melanoma, colon and lung cancer cell lines.Molecular cancer · 2013Article
- Unlaid Xenopus eggs degrade by apoptosis in the genital tract.BMC cell biology · 2013Article
- Unfertilized frog eggs die by apoptosis following meiotic exit.BMC cell biology · 2011Article
- Photodynamic treatment induces an apoptotic pathway involving calcium, nitric oxide, p53, p21-activated kinase 2, and c-Jun N-terminal kinase and inactivates survival signal in human umbilical vein endothelial cells.International journal of molecular sciences · 2011Article
- PAK thread from amoeba to mammals.Journal of cellular biochemistry · 2009Review
- p42MAPK-mediated phosphorylation of xEIAP/XLX in Xenopus cytostatic factor-arrested egg extracts.BMC biochemistry · 2007Article
- EphA4 signaling regulates blastomere adhesion in the Xenopus embryo by recruiting Pak1 to suppress Cdc42 function.Molecular biology of the cell · 2007Article
- GCK-3, a newly identified Ste20 kinase, binds to and regulates the activity of a cell cycle-dependent ClC anion channel.The Journal of general physiology · 2005Article
- p38 Mitogen-activated protein kinase mediates cell death and p21-activated kinase mediates cell survival during chemotherapeutic drug-induced mitotic arrest.Molecular biology of the cell · 2003Article
- XPak3 promotes cell cycle withdrawal during primary neurogenesis in Xenopus laevis.The EMBO journal · 2002Article
- Mating-type-specific and nonspecific PAK kinases play shared and divergent roles in Cryptococcus neoformans.Eukaryotic cell · 2002Article
- Post-cytochrome C protection from apoptosis conferred by a MAPK pathway in Xenopus egg extracts.Molecular biology of the cell · 2002Article
- A novel p21-activated kinase binds the actin and microtubule networks and induces microtubule stabilization.The Journal of cell biology · 2001Article
- Regulation of the p21-activated kinase (PAK) by a human Gbeta -like WD-repeat protein, hPIP1.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Functional interaction between c-Abl and the p21-activated protein kinase gamma-PAK.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- MHC class II pseudogene and genomic signature of a 32-kb cosmid in the house finch (Carpodacus mexicanus).Genome research · 2000Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have identified new members (X-PAKs) of the Ste20/PAK family of protein kinases in Xenopus, and investigated their role in the process that maintains oocytes arrested in the cell cycle. Microinjection of a catalytically inactive mutant of X-PAK1 with a K/R substitution in the ATP binding site, also deleted of its Nter-half that contains the conserved domains responsible for binding of both Cdc42/Rac GTPases and SH3-containing proteins, greatly facilitates oocyte release from G2/prophase arrest by progesterone and insulin. Addition of the same X-PAK1 mutant to cell cycle extracts from unfertilized eggs induced apoptosis, as shown by activation of caspases and cytological changes in in vitro-assembled nuclei. This was suppressed by adding Bcl-2 or the DEVD peptide inhibitor of caspases, and rescued by competing the dominant-negative mutant with its constitutively active X-PAK1 counterpart. Such results indicate that X-PAK1 (or another member of the Xenopus Ste20/PAK family of protein kinases) is involved in arrest of oocytes at G2/prophase and prevention of apoptosis; thus death by apoptosis and release of healthy oocytes from cell cycle arrest may be linked. That cell cycle arrest protects oocytes from apoptosis is consistent with the finding that extracts from metaphase II-arrested oocytes are less sensitive to apoptotic signals than those from activated eggs.
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