ArticleThe EMBO journal1997
RNA recognition by the joint action of two nucleolin RNA-binding domains: genetic analysis and structural modeling.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- A translational repression reporter assay for the analysis of RNA-binding protein consensus sites.RNA biology · 2023Article
- Interaction of nucleolin with ribosomal RNA genes and its role in RNA polymerase I transcription.Nucleic acids research · 2012Article
- Interactions of nucleolin and ribosomal protein L26 (RPL26) in translational control of human p53 mRNA.The Journal of biological chemistry · 2012Article
- Nucleolin binds to a subset of selenoprotein mRNAs and regulates their expression.Nucleic acids research · 2010Article
- Identification and characterization of nucleolin as a c-myc G-quadruplex-binding protein.The Journal of biological chemistry · 2009Article
- The adaptor Grb7 links netrin-1 signaling to regulation of mRNA translation.The EMBO journal · 2007Article
- Subnucleolar location of fibrillarin and NopA64 in Lepidium sativum root meristematic cells is changed in altered gravity.Protoplasma · 2006Article
- The N- and C-terminal RNA recognition motifs of splicing factor Prp24 have distinct functions in U6 RNA binding.RNA (New York, N.Y.) · 2005Article
- A novel conserved RNA-binding domain protein, RBD-1, is essential for ribosome biogenesis.Molecular biology of the cell · 2002Article
- RNA-binding strategies common to cold-shock domain- and RNA recognition motif-containing proteins.Nucleic acids research · 2001Article
- Molecular basis of sequence-specific recognition of pre-ribosomal RNA by nucleolin.The EMBO journal · 2000Article
- The carboxy-terminal fragment of nucleolin interacts with the nucleocapsid domain of retroviral gag proteins and inhibits virion assembly.Journal of virology · 2000Article
- UPA, a universal protein array system for quantitative detection of protein-protein, protein-DNA, protein-RNA and protein-ligand interactions.Nucleic acids research · 2000Article
- Separable roles in vivo for the two RNA binding domains of Drosophila A1-hnRNP homolog.RNA (New York, N.Y.) · 1998Article
- Nucleolin functions in the first step of ribosomal RNA processing.The EMBO journal · 1998Article
- Compensatory mutations in the L30e kink-turn RNA-protein complex.Biochimica et biophysica actaArticle
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interaction of nucleolin with a short stem-loop structure (NRE) requires two contiguous RNA-binding domains (RBD 1+2). The structural basis for RNA recognition by these RBDs was studied using a genetic system in Escherichia coli. Within each of the two domains, we identified several mutations that severely impair interaction with the RNA target. Mutations that alter RNA-binding specificity were also isolated, suggesting the identity of specific contacts between RBD 1+2 amino acids and nucleotides within the NRE stem-loop. Our data indicate that both RBDs participate in a joint interaction with the NRE and that each domain uses a different surface to contact the RNA. The constraints provided by these genetic data and previous mutational studies have enabled us to propose a three-dimensional model of nucleolin RBD 1+2 bound to the NRE stem-loop.
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