ArticleGenes & development1997
DNA methylation inhibits elongation but not initiation of transcription in Neurospora crassa.
Article in Genes & development, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
85 citing papers in PubMed, 215 citations in OpenAlex.
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- Fungi as models of centromere innovation: from DNA sequence to 3-dimensional arrangement.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Reading banned regions of genomes.Nature plants · 2024Article
- DDM1-mediated gene body DNA methylation is associated with inducible activation of defense-related genes in Arabidopsis.Genome biology · 2023Article
- Characterizing crosstalk in epigenetic signaling to understand disease physiology.The Biochemical journal · 2023Article
- Molecular mechanisms underlying host-induced gene silencing.The Plant cell · 2022Review
- Regulation of DNA methylation on key parasitism genes of Cysticercus cellulosae revealed by integrative epigenomic-transcriptomic analyses.Hereditas · 2021Article
- Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis.Nature communications · 2021Article
- Genome-Wide DNA Methylation Profile in Jejunum Reveals the Potential Genes Associated With Paratuberculosis in Dairy Cattle.Frontiers in genetics · 2021Article
- Article
- LSD1 prevents aberrant heterochromatin formation in Neurospora crassa.Nucleic acids research · 2020Article
- Machine learning analyses of methylation profiles uncovers tissue-specific gene expression patterns in wheat.The plant genome · 2020Article
- RIP mutated ITS genes in populations ofIMA fungus · 2020Article
- DNA Methylation: Shared and Divergent Features across Eukaryotes.Trends in genetics : TIG · 2019Review
- New guidelines for DNA methylome studies regarding 5-hydroxymethylcytosine for understanding transcriptional regulation.Genome research · 2019Article
- Neuroepigenetic mechanisms underlying fear extinction: emerging concepts.Psychopharmacology · 2019Review
- Consistent inverse correlation between DNA methylation of the first intron and gene expression across tissues and species.Epigenetics & chromatin · 2018Article
- Memory Function in Feeding Habit Transformation of Mandarin Fish (International journal of molecular sciences · 2018Article
- Human Skeletal Muscle Possesses an Epigenetic Memory of Hypertrophy.Scientific reports · 2018Article
- Acute Stress Enhances Epigenetic Modifications But Does Not Affect the Constitutive Binding of pCREB to Immediate-Early Gene Promoters in the Rat Hippocampus.Frontiers in molecular neuroscience · 2017Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In plants, animals, and fungi, DNA methylation is frequently associated with gene silencing, yet little is known about the role of the methylation in silencing. In Neurospora crassa, repeated sequences are silenced by repeat-induced point mutation (RIP) and genes that have suffered numerous GC --> AT mutations by RIP are typically methylated at remaining cytosines. We investigated possible effects on transcription from methylation associated with RIP by taking advantage of 5-azacytidine, which prevents most methylation in Neurospora and a dim-2 mutation that abolishes all detectable methylation. Northern analyses revealed that methylation prevents the accumulation of transcripts from genes mutated by RIP. Measurements of transcription rates in vivo showed that methylation inhibits transcription severely but does not influence mRNA stability. Results of nuclear run-on experiments demonstrated that transcription initiation was not significantly inhibited by the dense methylation in the promoter sequences. In contrast, methylation blocked transcription elongation in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.