ArticleMolecular biology of the cell1997
Wiskott-Aldrich syndrome protein is associated with the adapter protein Grb2 and the epidermal growth factor receptor in living cells.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 113 citations in OpenAlex.
- Small GTPase Cdc42, WASP, and scaffold proteins for higher-order assembly of the F-BAR domain protein.Science advances · 2023Article
- GRB2 dimerization mediated by SH2 domain-swapping is critical for T cell signaling and cytokine production.Scientific reports · 2023Article
- The Coordination Between B Cell Receptor Signaling and the Actin Cytoskeleton During B Cell Activation.Frontiers in immunology · 2018Review
- Multicohort analysis reveals baseline transcriptional predictors of influenza vaccination responses.Science immunology · 2017Article
- Allosteric N-WASP activation by an inter-SH3 domain linker in Nck.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Binding of the WASP/N-WASP-interacting protein WIP to actin regulates focal adhesion assembly and adhesion.Molecular and cellular biology · 2014Article
- Actin-mediated feedback loops in B-cell receptor signaling.Immunological reviews · 2013Review
- Article
- Models for actin polymerization motors.Journal of mathematical biology · 2009Review
- Recent advances in the biology of WASP and WIP.Immunologic research · 2009Review
- Grb2 signaling in cell motility and cancer.Expert opinion on therapeutic targets · 2008Review
- Rapid platelet turnover in WASP(-) mice correlates with increased ex vivo phagocytosis of opsonized WASP(-) platelets.Experimental hematology · 2008Article
- Growth factor receptor binding protein 2-mediated recruitment of the RING domain of Cbl to the epidermal growth factor receptor is essential and sufficient to support receptor endocytosis.Molecular biology of the cell · 2005Article
- Abelson-interactor-1 promotes WAVE2 membrane translocation and Abelson-mediated tyrosine phosphorylation required for WAVE2 activation.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2.Oncogene · 2004Article
- A neural-specific splicing event generates an active form of the Wiskott-Aldrich syndrome protein.EMBO reports · 2004Article
- Erk/Src phosphorylation of cortactin acts as a switch on-switch off mechanism that controls its ability to activate N-WASP.Molecular and cellular biology · 2004Article
- Signalling to actin assembly via the WASP (Wiskott-Aldrich syndrome protein)-family proteins and the Arp2/3 complex.The Biochemical journal · 2004Review
- Cellular prion protein promotes Brucella infection into macrophages.The Journal of experimental medicine · 2003Article
- Direct binding of a fragment of the Wiskott-Aldrich syndrome protein to the C-terminal end of the anaphylatoxin C5a receptor.The Biochemical journal · 2003Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Src homology domains [i.e., Src homology domain 2 (SH2) and Src homology domain 3 (SH3)] play a critical role in linking receptor tyrosine kinases to downstream signaling networks. A well-defined function of the SH3-SH2-SH3 adapter Grb2 is to link receptor tyrosine kinases, such as the epidermal growth factor receptor (EGFR), to the p21ras-signaling pathway. Grb2 has also been implicated to play a role in growth factor-regulated actin assembly and receptor endocytosis, although the underlying mechanisms remain unclear. In this study, we show that Grb2 interacts through its SH3 domains with the human Wiskott-Aldrich syndrome protein (WASp), which plays a role in regulation of the actin cytoskeleton. We find that WASp is expressed in a variety of cell types and is exclusively cytoplasmic. Although the N-terminal SH3 domain of Grb2 binds significantly stronger than the C-terminal SH3 domain to WASp, full-length Grb2 shows the strongest binding. Both phosphorylation of WASp and its interaction with Grb2, as well as with another adapter protein Nck, remain constitutive in serum-starved or epidermal growth factor-stimulated cells. WASp coimmunoprecipitates with the activated EGFR after epidermal growth factor stimulation. Purified glutathione S-transferase-full-length-Grb2 fusion protein, but not the individual domains of Grb2, enhances the association of WASp with the EGFR, suggesting that Grb2 mediates the association of WASp with EGFR. This study suggests that Grb2 translocates WASp from the cytoplasm to the plasma membrane and the Grb2-WASp complex may play a role in linking receptor tyrosine kinases to the actin cytoskeleton.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.