ArticleThe Biochemical journal1997
Evidence that human class Theta glutathione S-transferase T1-1 can catalyse the activation of dichloromethane, a liver and lung carcinogen in the mouse. Comparison of the tissue distribution of GST T1-1 with that of classes Alpha, Mu and Pi GST in human.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 2 of them syntheses that pooled it.
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Who cites it
26 citing papers in PubMed, 2 syntheses or guidelines pooled it, 151 citations in OpenAlex.
- GSTM1 and GSTT1 Null Genotype Polymorphisms and Susceptibility to Arsenic Poisoning: a Meta-analysis.Biological trace element research · 2021Pooled it
- An updating meta-analysis of the glutathione S-transferase T1 polymorphisms and colorectal cancer risk: a HuGE review.International journal of colorectal disease · 2010Pooled it
- Are glutathione S-transferase gene polymorphisms linked to neonatal jaundice?European journal of pediatrics · 2008Trial
- Article
- Single nucleotide polymorphisms and microsatellites in the canine glutathione S-transferase pi 1 (Canine genetics and epidemiology · 2017Article
- Spontaneous Production of Glutathione-Conjugated Forms of 1,2-Dichloropropane: Comparative Study on Metabolic Activation Processes of Dihaloalkanes Associated with Occupational Cholangiocarcinoma.Oxidative medicine and cellular longevity · 2017Article
- Benzene oxide is a substrate for glutathione S-transferases.Chemico-biological interactions · 2015Article
- Characterization of a Highly pH Stable Chi-Class Glutathione S-Transferase from Synechocystis PCC 6803.PloS one · 2015Article
- Characterization of affinity-purified isoforms of Acinetobacter calcoaceticus Y1 glutathione transferases.TheScientificWorldJournal · 2014Article
- Different carcinogenic process in cholangiocarcinoma cases epidemically developing among workers of a printing company in Japan.International journal of clinical and experimental pathology · 2014Article
- On enzyme-based anticancer molecular dietary manipulations.Journal of biomedicine & biotechnology · 2012Review
- Correlating of GSTM1, GSTT1, and GSTP1 genetic polymorphisms with the risk and expressions in children with isolated Hirschsprung disease.International journal of colorectal disease · 2011Article
- Glutathione s-transferases in pediatric cancer.Frontiers in oncology · 2011Article
- Genetic variations in human glutathione transferase enzymes: significance for pharmacology and toxicology.Human genomics and proteomics : HGP · 2010Article
- The 341C/T polymorphism in the GSTP1 gene is associated with increased risk of oesophageal cancer.BMC genetics · 2010Article
- Novel class of glutathione transferases from cyanobacteria exhibit high catalytic activities towards naturally occurring isothiocyanates.The Biochemical journal · 2007Article
- Polychlorinated biphenyls disturb differentiation of normal human neural progenitor cells: clue for involvement of thyroid hormone receptors.Environmental health perspectives · 2005Article
- Residue 234 in glutathione transferase T1-1 plays a pivotal role in the catalytic activity and the selectivity against alternative substrates.The Biochemical journal · 2005Article
- A human minor histocompatibility antigen resulting from differential expression due to a gene deletion.The Journal of experimental medicine · 2003Article
- Ure2, a prion precursor with homology to glutathione S-transferase, protects Saccharomyces cerevisiae cells from heavy metal ion and oxidant toxicity.The Journal of biological chemistry · 2003Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cDNA encoding human glutathione S-transferase (GST) T1 has been expressed as two recombinant forms in Escherichia coli that could be purified by affinity chromatography on either IgG-Sepharose or nickel-agarose; one form of the transferase was synthesized from the pALP 1 expression vector as a Staphylococcus aureus protein A fusion, whereas the other form was synthesized from the pET-20b expression vector as a C-terminal polyhistidine-tagged recombinant. The yields of the two purified recombinant proteins from E. coli cultures were approx. 15 mg/l for the protein A fusion and 25 mg/l for the C-terminal polyhistidine-tagged GST T1-1. The purified recombinant proteins were catalytically active, although the protein A fusion was typically only 5-30% as active as the histidine-tagged GST. Both recombinant forms could catalyse the conjugation of glutathione with the model substrates 1,2-epoxy-3-(4'-nitrophenoxy)propane,4-nitrobenzyl chloride and 4-nitrophenethyl bromide but were inactive towards 1-chloro-2,4-dinitrobenzene, ethacrynic acid and 1-menaphthyl sulphate. Recombinant human GST T1-1 was found to exhibit glutathione peroxidase activity and could catalyse the reduction of cumene hydroperoxide. In addition, recombinant human GST T1-1 was found to conjugate glutathione with dichloromethane, a pulmonary and hepatic carcinogen in the mouse. Immunoblotting with antibodies raised against different transferase isoenzymes showed that GST T1-1 is expressed in a large number of human organs in a tissue-specific fashion that differs from the pattern of expression of classes Alpha, Mu and Pi GST. Most significantly, GST T1-1 was found in only low levels in human pulmonary soluble extract of cells, suggesting that in man the lung has little capacity to activate the volatile dichloromethane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.