ArticleThe EMBO journal1997
Random mutagenesis reveals a region important for gating of the yeast K+ channel Ykc1.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Molecular architecture of the fungal-specific potassium channel TOK1.Nature communications · 2026Article
- Electrical signaling in fungi: past and present challenges.FEMS microbiology reviews · 2025Review
- TOK channels use the two gates in classical KFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Quantitative description of ion transport via plasma membrane of yeast and small cells.Frontiers in plant science · 2015Review
- Yeast luminometric and Xenopus oocyte electrophysiological examinations of the molecular mechanosensitivity of TRPV4.Journal of visualized experiments : JoVE · 2013Article
- A high-throughput functional screen identifies small molecule regulators of temperature- and mechano-sensitive K2P channels.ACS chemical biology · 2013Article
- Random mutagenesis of the proton-coupled folate transporter (SLC46A1), clustering of mutations, and the bases for associated losses of function.The Journal of biological chemistry · 2011Article
- Wild-type and brachyolmia-causing mutant TRPV4 channels respond directly to stretch force.The Journal of biological chemistry · 2010Article
- Forward genetic analysis reveals multiple gating mechanisms of TRPV4.The Journal of biological chemistry · 2010Article
- Alkali metal cation transport and homeostasis in yeasts.Microbiology and molecular biology reviews : MMBR · 2010Review
- Searching for interesting channels: pairing selection and molecular evolution methods to study ion channel structure and function.Molecular bioSystems · 2009Review
- Mutants of the Arabidopsis thaliana cation/H+ antiporter AtNHX1 conferring increased salt tolerance in yeast: the endosome/prevacuolar compartment is a target for salt toxicity.The Journal of biological chemistry · 2009Article
- Ion channels in microbes.Physiological reviews · 2008Review
- Functional consequences of leucine and tyrosine mutations in the dual pore motifs of the yeast K(+) channel, Tok1p.Pflugers Archiv : European journal of physiology · 2008Article
- A yeast genetic screen reveals a critical role for the pore helix domain in TRP channel gating.Neuron · 2008Article
- Yeast gain-of-function mutations reveal structure-function relationships conserved among different subfamilies of transient receptor potential channels.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Yeast screens show aromatic residues at the end of the sixth helix anchor transient receptor potential channel gate.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- An HSV vector system for selection of ligand-gated ion channel modulators.Nature methods · 2007Article
- In the yeast potassium channel, Tok1p, the external ring of aspartate residues modulates both gating and conductance.Pflugers Archiv : European journal of physiology · 2005Article
- Ability of Sit4p to promote K+ efflux via Nha1p is modulated by Sap155p and Sap185p.Eukaryotic cell · 2005Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
YKC1 (TOK1, DUK1, YORK) encodes the outwardly rectifying K+ channel of the yeast plasma membrane. Non-targeted mutations of YKC1 were isolated by their ability to completely block proliferation when expressed in yeast. All such mutations examined occurred near the cytoplasmic ends of the transmembrane segments following either of the duplicated P loops, which we termed the 'post-P loop' (PP) regions. These PP mutations specifically caused marked defects in the 'C1' states, a set of interrelated closed states that Ykc1 enters and exits at rates of tens to hundreds of milliseconds. These results indicate that the Ykc1 PP region plays a role in determining closed state conformations and that non-targeted mutagenesis and microbial selection can be a valuable tool for probing structure-function relationships of ion channels.
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