ArticleThe EMBO journal1997
Direct interaction of the calcium sensor protein synaptotagmin I with a cytoplasmic domain of the alpha1A subunit of the P/Q-type calcium channel.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 117 citations in OpenAlex.
- The high-affinity calcium sensor synaptotagmin-7 serves multiple roles in regulated exocytosis.The Journal of general physiology · 2018Review
- Phosphatidylinositol (4, 5)-bisphosphate targets double C2 domain protein B to the plasma membrane.Traffic (Copenhagen, Denmark) · 2017Article
- Synaptotagmin isoforms confer distinct activation kinetics and dynamics to chromaffin cell granules.The Journal of general physiology · 2017Article
- Synaptotagmin I delays the fast inactivation of Kv1.4 channel through interaction with its N-terminus.Molecular brain · 2014Article
- Physiology and pathology of calcium signaling in the brain.Frontiers in pharmacology · 2012Article
- Voltage-gated calcium channels.Cold Spring Harbor perspectives in biology · 2011Review
- Signaling complexes of voltage-gated sodium and calcium channels.Neuroscience letters · 2010Review
- Calcium-dependent regulation of SNARE-mediated membrane fusion by calmodulin.The Journal of biological chemistry · 2010Article
- Article
- Structure of human synaptotagmin 1 C2AB in the absence of Ca2+ reveals a novel domain association.Biochemistry · 2007Article
- Differential regulation of endogenous N- and P/Q-type Ca2+ channel inactivation by Ca2+/calmodulin impacts on their ability to support exocytosis in chromaffin cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Different effects on fast exocytosis induced by synaptotagmin 1 and 2 isoforms and abundance but not by phosphorylation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Functional maturation of nicotinic acetylcholine receptors as an indicator of murine muscular differentiation in a new nerve-muscle co-culture system.Pflugers Archiv : European journal of physiology · 2003Article
- Synaptotagmin function illuminated.The Journal of general physiology · 2003Review
- RGS3 mediates a calcium-dependent termination of G protein signaling in sensory neurons.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Ionic dependence of Ca2+ channel modulation by syntaxin 1A.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- The voltage-gated Ca2+ channel is the Ca2+ sensor of fast neurotransmitter release.Cellular and molecular neurobiology · 2001Article
- Ca2+-dependent regulation of synaptic SNARE complex assembly via a calmodulin- and phospholipid-binding domain of synaptobrevin.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Alternative splicing in the cytoplasmic II-III loop of the N-type Ca channel alpha 1B subunit: functional differences are beta subunit-specific.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Syntaxin modulation of calcium channels in cortical synaptosomes as revealed by botulinum toxin C1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synaptotagmins are synaptic vesicle proteins containing two calcium-binding C2 domains which are involved in coupling calcium influx through voltage-gated channels to vesicle fusion and exocytosis of neurotransmitters. The interaction of synaptotagmins with native P/Q-type calcium channels was studied in solubilized synaptosomes from rat cerebellum. Antibodies against synaptotagmins I and II, but not IV co-immunoprecipitated [125I]omega-conotoxin MVIIC-labelled calcium channels. Direct interactions were studied between in vitro-translated [35S]synaptotagmin I and fusion proteins containing cytoplasmic loops of the alpha1A subunit (BI isoform). Gel overlay revealed the association of synaptotagmin I with a single region (residues 780-969) located in the intracellular loop connecting homologous domains II and III. Saturable calcium-independent binding occurred with equilibrium dissociation constants of 70 nM and 340 nM at 4 degrees C and pH 7.4, and association was blocked by addition of excess recombinant synaptotagmin I. Direct synaptotagmin binding to the pore-forming subunit of the P/Q-type channel may optimally locate the calcium-binding sites that initiate exocytosis within a zone of voltage-gated calcium entry.
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