ArticleAnalytical biochemistry1997
Synthesis and spectral characterization of a thiol-reactive long-lifetime Ru(II) complex.
Article in Analytical biochemistry, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Ruthenium Complexes in Protein Science: Heading a New Generation of Therapeutic and Analytical Tools.Angewandte Chemie (International ed. in English) · 2026Review
- Long-distance tmFRET using bipyridyl- and phenanthroline-based ligands.Biophysical journal · 2024Article
- Long-distance tmFRET using bipyridyl- and phenanthroline-based ligands.bioRxiv : the preprint server for biology · 2024Article
- Nanodiscs: A Controlled Bilayer Surface for the Study of Membrane Proteins.Annual review of biophysics · 2018Article
- Ru(II)-diimine functionalized metalloproteins: From electron transfer studies to light-driven biocatalysis.Biochimica et biophysica acta · 2016Review
- A facile and versatile methodology for cysteine specific labeling of proteins with octahedral polypyridyl d⁶ metal complexes.Journal of inorganic biochemistry · 2014Article
- Fluorescent Metal Nanoshells: Lifetime-Tunable Molecular Probes in Fluorescent Cell Imaging.The journal of physical chemistry. C, Nanomaterials and interfaces · 2011Article
- Dynamics of bacteriophage R17 probed with a long-lifetime Ru(II) metal-ligand complex.Journal of fluorescence · 2010Article
- High-molecular-weight protein hydrodynamics studied with a long-lifetime metal-ligand complex.Biochimica et biophysica acta · 2002Article
- Lateral diffusion coefficients in membranes measured by resonance energy transfer and a new algorithm for diffusion in two dimensions.Biophysical journal · 2002Article
- End-to-end diffusion on the microsecond timescale measured with resonance energy transfer from a long-lifetime rhenium metal-ligand complex.Photochemistry and photobiology · 2000Article
- Long-lifetime lipid rhenium metal-ligand complex for probing membrane dynamics on the microsecond timescale.Chemistry and physics of lipids · 1999Article
- Synthesis and characterization of a sulfhydryl-reactive rhenium metal-ligand complex.Bioconjugate chemistryArticle
Corrections and comments
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4 authors.
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Abstract
We report the synthesis and spectral properties of a long-lifetime luminescent Ru complex containing a sulfhydryl-reactive maleimide group, [Ru (2,2'-bipyridine)2(1, 10-phenanthroline-5-maleimide)](PF6)2. [Ru(bpy)2(phen-mi)]2+ was covalently linked to human serum albumin, immunoglobulin G, and beta-galactosidase. The lifetimes for probe bound to proteins were near 1.1 micros. In the absence of rotational motions, the probe displayed an anisotropy near 0.17 for excitation near 475 nm. Anisotropy decay data were used to determine rotational correlation times of the proteins, which showed local probe motions in addition to overall rotational diffusion. This long-lifetime sulfhydryl-reactive probe can be used to recover microsecond rotational motions and/or domain motions of proteins and/or macromolecular complexes.
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