ArticleThe Journal of cell biology1997
Expression of TRPC3 in Chinese hamster ovary cells results in calcium-activated cation currents not related to store depletion.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed, 256 citations in OpenAlex.
- The role of IPPflugers Archiv : European journal of physiology · 2026Review
- Utilizing native nanodiscs to isolate active TRPC3 channels and expand structural analysis capabilities.Scientific reports · 2025Article
- Increased luminal pressure in brain capillaries drives TRPC3-dependent depolarization and constriction of transitional pericytes.Science signaling · 2025Article
- Review
- Regulatory mechanisms controlling store-operated calcium entry.Frontiers in physiology · 2023Review
- Role of Store-Operated CaBiomolecules · 2021Review
- Novel Mechanistic Insights and Potential Therapeutic Impact of TRPC6 in Neurovascular Coupling and Ischemic Stroke.International journal of molecular sciences · 2021Review
- Review
- TRPC Channels in the SOCE Scenario.Cells · 2020Review
- Article
- Protective effects of glycerol and xylitol in keratinocytes exposed to hyperosmotic stress.Clinical, cosmetic and investigational dermatology · 2019Article
- Mechanistic insights into the detection of free fatty and bile acids by ileal glucagon-like peptide-1 secreting cells.Molecular metabolism · 2018Article
- IPCellular and molecular life sciences : CMLS · 2017Review
- Smooth Muscle Ion Channels and Regulation of Vascular Tone in Resistance Arteries and Arterioles.Comprehensive Physiology · 2017Review
- PERK regulates GMolecular brain · 2016Article
- Article
- TRPC6 is the endothelial calcium channel that regulates leukocyte transendothelial migration during the inflammatory response.The Journal of experimental medicine · 2015Article
- TRPC3-dependent synaptic transmission in central mammalian neurons.Journal of molecular medicine (Berlin, Germany) · 2015Review
- Calcium activates the light-dependent conductance in melanopsin-expressing photoreceptors of amphioxus.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Transient receptor potential channels in the vasculature.Physiological reviews · 2015Review
22 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
TRPC3 (or Htrp3) is a human member of the trp family of Ca2+-permeable cation channels. Since expression of TRPC3 cDNA results in markedly enhanced Ca2+ influx in response to stimulation of membrane receptors linked to phospholipase C (Zhu, X., J. Meisheng, M. Peyton, G. Bouley, R. Hurst, E. Stefani, and L. Birnbaumer. 1996. Cell. 85:661-671), we tested whether TRPC3 might represent a Ca2+ entry pathway activated as a consequence of depletion of intracellular calcium stores. CHO cells expressing TRPC3 after intranuclear injection of cDNA coding for TRPC3 were identified by fluorescence from green fluorescent protein. Expression of TRPC3 produced cation currents with little selectivity for Ca2+ over Na+. These currents were constitutively active, not enhanced by depletion of calcium stores with inositol-1,4,5-trisphosphate or thapsigargin, and attenuated by strong intracellular Ca2+ buffering. Ionomycin led to profound increases of currents, but this effect was strictly dependent on the presence of extracellular Ca2+. Likewise, infusion of Ca2+ into cell through the patch pipette increased TRPC3 currents. Therefore, TRPC3 is stimulated by a Ca2+-dependent mechanism. Studies on TRPC3 in inside-out patches showed cation-selective channels with 60-pS conductance and short (<2 ms) mean open times. Application of ionomycin to cells increased channel activity in cell-attached patches. Increasing the Ca2+ concentration on the cytosolic side of inside-out patches (from 0 to 1 and 30 microM), however, failed to stimulate channel activity, even in the presence of calmodulin (0.2 microM). We conclude that TRPC3 codes for a Ca2+-permeable channel that supports Ca2+-induced Ca2+-entry but should not be considered store operated.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.