ArticleThe Journal of cell biology1997
Calcium release at fertilization in starfish eggs is mediated by phospholipase Cgamma.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 137 citations in OpenAlex.
- Article
- Calcium signaling in oocyte quality and functionality and its application.Frontiers in endocrinology · 2024Review
- Modulators of calcium signalling at fertilization.Open biology · 2020Review
- Insemination or phosphatidic acid induces an outwardly spiraling disk of elevated CaDevelopmental biology · 2019Article
- The eggstraordinary story of how life begins.Molecular reproduction and development · 2019Review
- Uncovering the genetic basis for early isogamete differentiation: a case study of Ectocarpus siliculosus.BMC genomics · 2013Article
- Calcium signalling in early embryos.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2008Review
- Calcium at fertilization and in early development.Physiological reviews · 2006Review
- Kinase activation through dimerization by human SH2-B.Molecular and cellular biology · 2005Article
- Tr-kit-induced resumption of the cell cycle in mouse eggs requires activation of a Src-like kinase.The EMBO journal · 2002Article
- Changes in organization of the endoplasmic reticulum during Xenopus oocyte maturation and activation.Molecular biology of the cell · 2001Article
- Calcium dynamics during fertilization in C. elegans.BMC developmental biology · 2001Article
- Inositol 1,4,5-trisphosphate directs Ca(2+) flow between mitochondria and the Endoplasmic/Sarcoplasmic reticulum: a role in regulating cardiac autonomic Ca(2+) spiking.Molecular biology of the cell · 2000Article
- Involvement of phospholipase Cgamma1 in mouse egg activation induced by a truncated form of the C-kit tyrosine kinase present in spermatozoa.The Journal of cell biology · 1998Article
- Protein tyrosine kinase signaling during oocyte maturation and fertilization.Molecular reproduction and developmentReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Although inositol trisphosphate (IP3) functions in releasing Ca2+ in eggs at fertilization, it is not known how fertilization activates the phospholipase C that produces IP3. To distinguish between a role for PLCgamma, which is activated when its two src homology-2 (SH2) domains bind to an activated tyrosine kinase, and PLCbeta, which is activated by a G protein, we injected starfish eggs with a PLCgamma SH2 domain fusion protein that inhibits activation of PLCgamma. In these eggs, Ca2+ release at fertilization was delayed, or with a high concentration of protein and a low concentration of sperm, completely inhibited. The PLCgammaSH2 protein is a specific inhibitor of PLCgamma in the egg, since it did not inhibit PLCbeta activation of Ca2+ release initiated by the serotonin 2c receptor, or activation of Ca2+ release by IP3 injection. Furthermore, injection of a PLCgamma SH2 domain protein mutated at its phosphotyrosine binding site, or the SH2 domains of another protein (the phosphatase SHP2), did not inhibit Ca2+ release at fertilization. These results indicate that during fertilization of starfish eggs, activation of phospholipase Cgamma by an SH2 domain-mediated process stimulates the production of IP3 that causes intracellular Ca2+ release.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.