ArticleJournal of bacteriology1997
Identification and nitrogen regulation of the cyanase gene from the cyanobacteria Synechocystis sp. strain PCC 6803 and Synechococcus sp. strain PCC 7942.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Deletion of the gene for a cyanobacterial ribosome-associated protein affects the carbon/nitrogen metabolism.bioRxiv : the preprint server for biology · 2026Article
- From photosynthetic electron flow to gene regulation: redox signal transduction in cyanobacteria.FEMS microbiology reviews · 2026Review
- Simultaneous acclimation to nitrogen and iron scarcity in open ocean cyanobacteria revealed by sparse tensor decomposition of metatranscriptomes.Science advances · 2025Article
- Nitrogen Journey in Plants: From Uptake to Metabolism, Stress Response, and Microbe Interaction.Biomolecules · 2023Review
- The genomes of Vischeria oleaginous microalgae shed light on the molecular basis of hyper-accumulation of lipids.BMC biology · 2023Article
- Site-2 Protease Slr1821 Regulates Carbon/Nitrogen Homeostasis during Ammonium Stress Acclimation in CyanobacteriumInternational journal of molecular sciences · 2023Article
- Crystal structure of a thermophilic fungal cyanase and its implications on the catalytic mechanism for bioremediation.Scientific reports · 2021Article
- Cyanate Assimilation by the Alkaliphilic Cyanide-Degrading BacteriumInternational journal of molecular sciences · 2019Article
- A review on remediation of cyanide containing industrial wastes using biological systems with special reference to enzymatic degradation.World journal of microbiology & biotechnology · 2019Review
- Expression of the cyanobacterial enzyme cyanase increases cyanate metabolism and cyanate tolerance in Arabidopsis.Environmental science and pollution research international · 2017Article
- Response of Prochlorococcus to varying CO2:O2 ratios.The ISME journal · 2015Article
- Biochemical and structural properties of cyanases from Arabidopsis thaliana and Oryza sativa.PloS one · 2011Article
- Characterization of cyanate metabolism in marine Synechococcus and Prochlorococcus spp.Applied and environmental microbiology · 2011Article
- Nitrite transport activity of the ABC-type cyanate transporter of the cyanobacterium Synechococcus elongatus.Journal of bacteriology · 2009Article
- Characterization of the Pseudomonas pseudoalcaligenes CECT5344 Cyanase, an enzyme that is not essential for cyanide assimilation.Applied and environmental microbiology · 2008Article
- Long-term response toward inorganic carbon limitation in wild type and glycolate turnover mutants of the cyanobacterium Synechocystis sp. strain PCC 6803.Plant physiology · 2007Article
- Involvement of the cynABDS operon and the CO2-concentrating mechanism in the light-dependent transport and metabolism of cyanate by cyanobacteria.Journal of bacteriology · 2007Article
- Computational inference and experimental validation of the nitrogen assimilation regulatory network in cyanobacterium Synechococcus sp. WH 8102.Nucleic acids research · 2006Article
- Comparative genomics analysis of NtcA regulons in cyanobacteria: regulation of nitrogen assimilation and its coupling to photosynthesis.Nucleic acids research · 2005Article
- Role of NtcB in activation of nitrate assimilation genes in the cyanobacterium Synechocystis sp. strain PCC 6803.Journal of bacteriology · 2001Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An open reading frame (slr0899) on the genome of Synechocystis sp. strain PCC 6803 encodes a polypeptide of 149 amino acid residues, the sequence of which is 40% identical to that of cyanase from Escherichia coli. Introduction into a cyanase-deficient E. coli strain of a plasmid-borne slr0899 resulted in expression of low but significant activity of cyanase. Targeted interruption of a homolog of slr0899 from Synechococcus sp. strain PCC 7942, encoding a protein 77% identical to that encoded by slr0899, resulted in loss of cellular cyanase activity. These results indicated that slr0899 and its homolog in the strain PCC 7942 represent the cyanobacterial cyanase gene (designated cynS). While cynS of strain PCC 6803 is tightly clustered with the four putative molybdenum cofactor biosynthesis genes located downstream, cynS of strain PCC 7942 was found to be tightly clustered with the two genes located upstream, which encode proteins similar to the subunits of the cyanobacterial nitrate-nitrite transporter. In both strains, cynS was transcribed as a part of a large transcription unit and the transcription was negatively regulated by ammonium. Cyanase activity was low in ammonium-grown cells and was induced 7- to 13-fold by inhibition of ammonium fixation or by transfer of the cells to ammonium-free media. These findings indicated that cyanase is an ammonium-repressible enzyme in cyanobacteria, the expression of which is regulated at the level of transcription. Similar to other ammonium-repressible genes in cyanobacteria, expression of cynS required NtcA, a global nitrogen regulator of cyanobacteria.
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