ArticleJournal of bacteriology1997
A specific protease encoded by the conjugative DNA transfer systems of IncP and Ti plasmids is essential for pilus synthesis.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 51 citations in OpenAlex.
- The cryo-EM structure of bacteriophage PRR1 and its role in conjugation inhibition.Journal of virology · 2026Article
- Phospholipid-independent biogenesis and function of the RP4 conjugation pilus.Nature communications · 2026Article
- Suppressing Transfer of Antibiotic Resistance by a Small RNA Virus.bioRxiv : the preprint server for biology · 2026Article
- Phospholipid-independent biogenesis of a functional RP4 conjugation pilus.bioRxiv : the preprint server for biology · 2025Article
- A comprehensive list of genes required for the efficient conjugation of plasmid Rts1 was determined by systematic deletion analysis.DNA research : an international journal for rapid publication of reports on genes and genomes · 2024Article
- Conjugative plasmids inhibit extracellular electron transfer inFrontiers in microbiology · 2023Article
- Structural determinants for peptide-bond formation by asparaginyl ligases.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Butelase 1 is an Asx-specific ligase enabling peptide macrocyclization and synthesis.Nature chemical biology · 2014Article
- Processing and maturation of the pilin of the type IV secretion system encoded within the gonococcal genetic island.The Journal of biological chemistry · 2011Article
- Evidence for VirB4-mediated dislocation of membrane-integrated VirB2 pilin during biogenesis of the Agrobacterium VirB/VirD4 type IV secretion system.Journal of bacteriology · 2010Article
- Novel Tn4371-ICE like element in Ralstonia pickettii and genome mining for comparative elements.BMC microbiology · 2009Article
- The structural biology of type IV secretion systems.Nature reviews. Microbiology · 2009Review
- Increased excision of the Salmonella prophage ST64B caused by a deficiency in Dam methylase.Journal of bacteriology · 2005Article
- The VirB4 family of proposed traffic nucleoside triphosphatases: common motifs in plasmid RP4 TrbE are essential for conjugation and phage adsorption.Journal of bacteriology · 2003Article
- Functional and mutational analysis of conjugative transfer region 2 (Tra2) from the IncHI1 plasmid R27.Journal of bacteriology · 2003Article
- Complete nucleotide sequence of plasmid Rts1: implications for evolution of large plasmid genomes.Journal of bacteriology · 2002Article
- Comparative sequence analysis of the symbiosis island of Mesorhizobium loti strain R7A.Journal of bacteriology · 2002Article
- Biogenesis of T pili in Agrobacterium tumefaciens requires precise VirB2 propilin cleavage and cyclization.Journal of bacteriology · 2002Article
- Maturation of IncP pilin precursors resembles the catalytic Dyad-like mechanism of leader peptidases.Journal of bacteriology · 2000Article
- The complete DNA sequence and analysis of R27, a large IncHI plasmid from Salmonella typhi that is temperature sensitive for transfer.Nucleic acids research · 2000Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
TraF, an essential component of the conjugative transfer apparatus of the broad-host-range plasmid RP4 (IncP), which is located at the periplasmic side of the cytoplasmic membrane, encodes a specific protease. The traF gene products of IncP and Ti plasmids show extensive similarities to prokaryotic and eukaryotic signal peptidases. Mutational analysis of RP4 TraF revealed that the mechanism of the proteolytic cleavage reaction resembles that of signal and LexA-like peptidases. Among the RP4 transfer functions, the product of the Tra2 gene, trbC, was identified as a target for the TraF protease activity. TrbC is homologous to VirB2 of Ti plasmids and thought to encode the RP4 prepilin. The maturation of TrbC involves three processing reactions: (i) the removal of the N-terminal signal peptide by Escherichia coli signal peptidase I (Lep), (ii) a proteolytic cleavage at the C terminus by an as yet unidentified host cell enzyme, and (iii) C-terminal processing by TraF. The third reaction of the maturation process is critical for conjugative transfer, pilus synthesis, and the propagation of the donor-specific bacteriophage PRD1. Thus, cleavage of TrbC by TraF appears to be one of the initial steps in a cascade of processes involved in export of the RP4 pilus subunit and pilus assembly mediated by the RP4 mating pair formation function.
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