Evidence map›Paper›PMID 9294215›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America1997

The epitopes for some antiphospholipid antibodies are adducts of oxidized phospholipid and beta2 glycoprotein 1 (and other proteins).

S Hörkkö, E Miller, D W Branch, W Palinski, J L Witztum

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PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
10.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 139 citations in OpenAlex.

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  18. Antiphospholipid antibodies: paradigm in transition.Journal of neuroinflammation · 2009
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

S HörkköDepartment of Medicine, University of California, San Diego, La Jolla, CA 92093-0682, USA.
E Miller
D W Branch
W Palinski
J L Witztum
University of Utah · US

Funding

ANTICARDIOLIPIN ANTIBODIES AND OXIDIZED PHOSPHOLIPIDSR01HL057505 · NHLBI · UNIVERSITY OF CALIFORNIA SAN DIEGO · PI WITZTUM, JOSEPH L. · 1997 to 2004
$2.0M
NHLBI NIH HHS HL57505NHLBI NIH HHS R01 HL057505PHS HHS 14197
6 · The paper itself

Abstract

Circulating autoantibodies to phospholipids (aPLs), such as cardiolipin (CL), are found in patients with antiphospholipid antibody syndrome (APS). We recently demonstrated that many aPLs bound to CL only after it had been oxidized (OxCL), but not to a reduced CL analogue that could not undergo oxidation. We now show that the neoepitopes recognized by some aPLs consist of adducts formed between breakdown products of oxidized phospholipid and associated proteins, such as beta2 glycoprotein 1 (beta2GP1). Addition of human beta2GP1, polylysine, native low-density lipoprotein, or apolipoprotein AI to OxCL-coated wells increased the anticardiolipin antibody (aCL) binding from APS sera that first had been diluted so that no aCL binding to OxCL could be detected. No increase in aCL binding was observed when these proteins were added to wells coated with reduced CL. The ability of beta2GP1, polylysine, or low-density lipoprotein to be a "cofactor" for aCL binding to OxCL was greatly reduced when the proteins were methylated. Incubation of beta2GP1 with oxidized 1-palmitoyl-2-linoleyl-[1-14C]-phosphatidylcholine (PC), but not with dipalmitoyl-[1-14C]-PC, led to formation of covalent adducts with beta2GP1 recognized by APS sera. These data suggest that the reactive groups of OxCL, such as aldehydes generated during the decomposition of oxidized polyunsaturated fatty acids, form covalent adducts with beta2GP1 (and other proteins) and that these are epitopes for aCLs. Knowledge that the epitopes recognized by many aPLs are adducts of oxidized phospholipid and associated proteins, including beta2GP1, may give new insights into the pathogenic events underlying the clinical manifestations of APS.

Indexed as

Antibodies, AntiphospholipidAntiphospholipid Syndromebeta 2-Glycoprotein IBinding Sites, AntibodyCarbon RadioisotopesCardiolipinsEpitopesFemaleGlycoproteinsHumansImmunoglobulin GMethylationOxidation-ReductionPhospholipidsProtein BindingAntibodies, Antiphospholipidbeta 2-Glycoprotein ICarbon RadioisotopesCardiolipinsEpitopesGlycoproteinsImmunoglobulin GPhospholipids

Identifiers

PMID9294215
PMCPMC23367
OpenAlexW2058954135

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.