ArticleThe Journal of experimental medicine1997
Rho is required for the initiation of calcium signaling and phagocytosis by Fcgamma receptors in macrophages.
Article in The Journal of experimental medicine, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
53 citing papers in PubMed, 165 citations in OpenAlex.
- Dexamethasone targets actin cytoskeleton signaling and inflammatory mediators to reverse sulfur mustard-induced toxicity in rabbit corneas.Toxicology and applied pharmacology · 2024Article
- Convergence of Ras- and Rac-regulated formin pathways is pivotal for phagosome formation and particle uptake inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Phagocytosis depends on TRPV2-mediated calcium influx and requires TRPV2 in lipids rafts: alteration in macrophages from patients with cystic fibrosis.Scientific reports · 2018Article
- Syncytium calcium signaling and macrophage function in the heart.Cell & bioscience · 2018Review
- Regulation of Microglial Phagocytosis by RhoA/ROCK-Inhibiting Drugs.Cellular and molecular neurobiology · 2017Article
- Altered macrophagic THP-1 cell phagocytosis and migration in bisphosphonate-related osteonecrosis of the jaw (BRONJ).Clinical oral investigations · 2016Article
- Macrophages phagocytose nonopsonized silica particles using a unique microtubule-dependent pathway.Molecular biology of the cell · 2015Article
- Coxiella burnetii Phagocytosis Is Regulated by GTPases of the Rho Family and the RhoA Effectors mDia1 and ROCK.PloS one · 2015Article
- Generation of membrane structures during phagocytosis and chemotaxis of macrophages: role and regulation of the actin cytoskeleton.Immunological reviews · 2013Review
- Multimolecular signaling complexes enable Syk-mediated signaling of CD36 internalization.Developmental cell · 2013Article
- Endothelium-neutrophil interactions in ANCA-associated diseases.Journal of the American Society of Nephrology : JASN · 2012Review
- Macrophage phagocytosis: effects of environmental pollutants, alcohol, cigarette smoke, and other external factors.Journal of leukocyte biology · 2011Review
- Regulation of tyrosine phosphorylation in macrophage phagocytosis and chemotaxis.Archives of biochemistry and biophysics · 2011Review
- DNA attenuates enterocyte Toll-like receptor 4-mediated intestinal mucosal injury after remote trauma.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- RhoB loss prevents streptozotocin-induced diabetes and ameliorates diabetic complications in mice.The American journal of pathology · 2011Article
- Leukotriene B4 augments and restores Fc gammaRs-dependent phagocytosis in macrophages.The Journal of biological chemistry · 2010Article
- Mechanisms of failed apoptotic cell clearance by phagocyte subsets in cardiovascular disease.Apoptosis : an international journal on programmed cell death · 2010Review
- Clostridium difficile toxin B differentially affects GPCR-stimulated Ca2+ responses in macrophages: independent roles for Rho and PLA2.Journal of leukocyte biology · 2010Article
- TRPV2 has a pivotal role in macrophage particle binding and phagocytosis.Nature immunology · 2010Article
- Dysfunctional cystic fibrosis transmembrane conductance regulator inhibits phagocytosis of apoptotic cells with proinflammatory consequences.American journal of physiology. Lung cellular and molecular physiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phagocytosis of bacteria by macrophages and neutrophils is an essential component of host defense against infection. The mechanism whereby the interaction of opsonized particles with Fcgamma receptors triggers the engulfment of opsonized particles remains incompletely understood, although activation of tyrosine kinases has been recognized as an early step. Recent studies in other systems have demonstrated that tyrosine kinases can in turn signal the activation of small GTPases of the ras superfamily. We therefore investigated the possible role of Rho in Fc receptor-mediated phagocytosis. To this end we microinjected J774 macrophages with C3 exotoxin from Clostridium botulinum, which ADP-ribosylates and inactivates Rho. C3 exotoxin induced the retraction of filopodia, the disappearance of focal complexes, and a global decrease in the F-actin content of J774 cells. In addition, these cells exhibited increased spreading and the formation of vacuolar structures. Importantly, inactivation of Rho resulted in the complete abrogation of phagocytosis. Inhibition of Fcgamma receptor-mediated phagocytosis by C3 exotoxin was confirmed in COS cells, which become phagocytic upon transfection of the FcgammaRIIA receptor. Rho was found to be essential for the accumulation of phosphotyrosine and of F-actin around phagocytic cups and for Fcgamma receptor-mediated Ca2+ signaling. The clustering of receptors in response to opsonin, an essential step in Fcgamma-induced signaling, was the earliest event shown to be inhibited by C3 exotoxin. The effect of the toxin was specific, since clustering and internalization of transferrin receptors were unaffected by microinjection of C3. These data identify a role for small GTPases in Fcgamma receptor-mediated phagocytosis by leukocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.